The NEBNext UltraExpress RNA Library Prep Kit is the latest generation of NEBNext RNA library prep, and it has a fast, streamlined workflow. With a 3-hour library prep protocol, the kit enables creation of high-quality RNA libraries in a single day, in conjunction with mRNA enrichment or rRNA depletion kits.
Fast workflow (3 hours)
Fewer steps and consumables
Single protocol for all input amounts
High-quality libraries from a broad input range: 25 - 250 ng total RNA
View or download extensive performance data below or in our Data Supplement.
This new kit meets the need for substantially faster, more streamlined RNA library prep workflows that deliver high-quality libraries from a variety of sample types in a single day from total RNA.
The kit allows the use of a single protocol for all RNA inputs (25 -250 ng total RNA) and incorporates master mixed reagents, reduced incubation times and fewer cleanup steps, reducing total time and consumables used.
3-hour library prep enables single day total RNA to library, in conjunction with mRNA enrichment or rRNA depletion kits
Fewer cleanups and pipetting steps reduce hands-on time
A robust single protocol for all input amounts increases ease of use
Flexibility is enabled with simple guidelines for customized protocols, if desired
Automation-friendly, enabling scalability
This product is related to the following categories:
NEBNext Multiplex Oligos options can be found at www.neb.com/oligos. Alternatively, customer supplied adaptor and primers can be used; please see information in link: https://www.neb.com/faqs/2019/03/08/can-i-use-this-nebnext-kit-with-adaptors-and-primers-from-other-vendors-than-neb
SPRIselect™ Reagent Kit (Beckman Coulter®, Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
Magnetic Rack (NEB S1515S, Alpaqua® cat. #A001322, or equivalent)
80% Ethanol (freshly prepared)
DNase-, RNase-free PCR strip tubes, for example TempAssure® PCR flex-free 8-tube strips (USA Scientific® #1402-4708)
Bioanalyzer® or TapeStation® (Agilent® Technologies, Inc.) and associated reagents and consumables
For use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490):
1.5 ml Microcentrifuge tube and NEB #S1506 Magnet stand or equivalent (for washing beads only)
For use with NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400) and other NEBNext RNA depletion kits that do not include beads (NEB #E7750, E7850, E7865):
Agencourt® RNAClean® XP Beads (Beckman Coulter, Inc. #A63987)
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