AbaSI

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
R0665S     -20       AbaSI R0665SVIAL -20 1 x 0.1 ml 10,000 units/ml   rCutSmart™ Buffer B6004SVIAL -20 1 x 1.25 ml 10 X   DTT B0706SVIAL -20 1 x 1 ml 0.4 M

Companion Products

• T4 Phage β-glucosyltransferase (T4-BGT)
• MspJI

1. Optimization of the amount of enzyme for complete digestion of genomic DNA may be required.
2. Not sensitive to CpG, dcm, or dam methylation.

1. Protocol for Glucosylation and digestion of Genomic DNA using AbaSI (#R0665)
2. Restriction Digest Protocol
3. Double Digest Protocol with Standard Restriction Enzymes

• Restriction Enzyme Tips

• Isoelectric Points (pI) for Restriction Enzymes
• NEB Diluent and Buffer Table

• Double Digest Finder
• Enzyme Finder
• NEBcutter™ v3.0
• NEBioCalculator®
• REBASE®

1. Does AbaSI cut hemi-glucosylated CpG site?
2. How do I know my DNA is cut?
3. How much enzyme should be used in digesting genomic DNA?
4. Is this enzyme sensitive to dam, dcm or mammalian CpG methylation?
5. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?

• Horton JR, Borgaro JG, Griggs RM, Quimby A, Guan S, Zhang X, Wilson GG, Zheng Y, Zhu Z, Cheng X (2014) Structure of 5-hydroxymethylcytosine-specific restriction enzyme AbaSI in complex with DNA Nucleic Acids Res; 42(12), 7947-59. PubMedID: 24895434, DOI: 10.1093/nar/gku497

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• R0665S_v3

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• R0665S_L_v1_0021304
• R0665S_v1_0021304
• R0665S_v1_0021403
• R0665S_v1_0021502
• R0665S_v1_0021507
• R0665S_v1_0021601
• R0665S_v2_0021605
• R0665S_v1_0021605
• R0665S_v2_0021612
• R0665S_v2_0021802
• R0665S_v2_10030066
• R0665S_v2_10053618
• R0665S_v2_10065377
• R0665S_v2_10097550
• R0665S_v3_10118491
• R0665S_v3_10163211
• R0665S_v3_10173517
• R0665S_v3_10197877
• R0665S_v3_10205103

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• AbaSI
• rCutSmart™ Buffer
• DTT

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.