Home DNA Modifying Enzymes and Cloning Technologies ET SSB

ET SSB
recombinant Gray property icon for heat inactivation no

ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

  • Improve the processivity of DNA polymerase
  • Stabilization and marking of ssDNA structure
  • Increase the yield and specificitiy of PCR
  • Increase the yield and processivity of RT during RT-PCR
  • Improve DNA sequencing through regions with strong secondary structure
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Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
M2401S 500 µg/ml 50 µg $190.00 $190.00
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  • ET SSB (Extreme Thermostable Single-Stranded DNA Binding Protein) is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95°C for 60 minutes. Due to the extreme thermostability, ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

    Figure 1: Improved multiplex PCR by ET SSB. Figure 1: improved multiplex PCR by ET SSB

    By adding ET SSB, specific amplification was accomplished in PCR using one to five primer pairs in incurring order (lanes 1-5). Lane 5C shows a PCR reaction using 5 primer pairs, with no ET SSB included (control reaction). Lane M: 100 bp DNA Ladder.
    Figure 2: Multiplex HDA was improved by ET SSB. Figure 2: Multiplex HDA was improved by ET SSB

    The HDA reactions were prepared with either single or two sets of primers to amplify the invE (A) and/or invA (B) from Salmonella typhimurium genomic DNA. Lane M: Low Molecular DNA Ladder; Lane A: invE gene; Lane B: invA gene; Lane AB: invE and invA genes with no ET SSB present; Lane AB**: invE and invA genes with ET SSB in the HDA reaction. ** the reaction contained ET SSB.

    Product Source

    An E. coli strain that carries the cloned ssb gene from a hyperthermophilic organism
    This product is related to the following categories:
    ssDNA Binding Proteins

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      Sold by mass of pure protein as determined by OD280.

      Storage Buffer

      10 mM Tris-HCl
      100 mM KCl
      0.1 mM EDTA
      50% Glycerol
      pH 7.4 @ 25°C

      Heat Inactivation

      No

      Molecular Weight

      Apparent: 16 kDa

    • Product Notes

      1. ET SSB is active in any polymerase buffer. Add 200 ng of ET SSB per 50 µl reaction.

    • References

      1. Myers, T.W. and Romano, L.J. (1988). J. Biol. Chem. 263, 17006-17015.
      2. Reddy, M.S. (2000). Biochemistry. 39, 14250-14262.
      3. West, S.C., Cassuto, E. and Howard-Flanders, P. (1982). Mol. Gen. Genet. 186, 333-338.
      4. Goldmeyer, J., Kong, H., and Tang, W. (2007). J. Mol. Diagnostics. 9, 639-644.
      5. Delius, H., Mantell, N.J. and Alberts, B. (1972). J. Mol. Biol. 67, 341-350.
      6. Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1990). Nucl. Acids Res. 18, 1079.
      7. Chou, Q. (1992). Nucl. Acids Res. 20, 4371.
      8. Oshima, R.G. (1992). Biotechniques. 13, 188.
      9. Rapley, R. (1994). Mol. Biotechnol. 2, 295-298.
      10. Olszewski, M. et al. (2005). Mol. Cell Probes. 19, 203-205.
      11. Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
      12. Villalva, C. et al. (2001). Biotechniques. 31, 81-83, 86.

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