AluI Methyltransferase

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0220S     -20       AluI Methyltransferase M0220SVIAL -20 1 x 0.02 ml 5,000 units/ml   AluI Methyltransferase Reaction Buffer B0220SVIAL -20 1 x 1 ml 10 X   S-adenosylmethionine (SAM) B9003SVIAL -20 1 x 0.1 ml 32 mM

Materials Sold Separately

• S-adenosylmethionine (SAM)

1. Hoffman, J.L. (1986). Biochemistry. 25, 444-4449.

1. Recommended Protocol for 3H labeling of DNA
2. Recommended Protocol for Methylation of Genomic DNA

• AppNote Use of Blunt TA Ligase Master Mix for Adaptor Ligation to dA Tailed dsDNA Fragments

1. Is S-adenosylmethionine (SAM) supplied with the Methyltransferase?
2. What should be considered if the methylation is not going to completion?
3. Can AluI Methyltransferase be heat inactivated?
4. Does AluI Methyltransferase require MgCl2?
5. What is the molecular weight of AluI Methyltransferase?
6. Can AluI methylated DNA be used to transform E. coli?
7. Does methylation with AluI Methyltransferase block SacI restriction endonuclease?
8. Will any NEB methyltransferases (methylases) work on RNA?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0220S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0220S_v1_10016805
• M0220S_v1_10038566
• M0220S_v1_10040394
• M0220S_v1_10045947
• M0220S_v1_10052773
• M0220S_v1_10056365
• M0220S_v1_10065601
• M0220S_v1_10084144
• M0220S_v1_10098562
• M0220S_v1_10106701
• M0220S_v1_10151496
• M0220S_v1_10191261
• M0220S_v1_0081803
• M0220S_v1_10245565
• M0220S_v1_10253672
• M0220S_v2_10279058
• M0220S_v2_10309216
• M0220S_v2_10313855

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• AluI Methyltransferase
• AluI Methyltransferase Reaction Buffer
• S-adenosylmethionine (SAM)

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.