Home DNA Modifying Enzymes and Cloning Technologies EcoRI Methyltransferase

EcoRI Methyltransferase
Gray property icon for rCutSmart Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 37° Gray property icon for heat inactivation 65° Gray property icon for SAM

EcoRI Methyltransferase is now supplied with rCutSmart Buffer (12/1/2021) 

M0211EcoRIMethyl_thumb
  • EcoRI Methyltransferase modifies the internal adenine residue (N6) of the sequence GAATTC
  • Supplied with rCutSmart Buffer and 32 mM S-adenosylmethionine (SAM)
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Catalog # Cat # Concentration Conc Size List Price List Price Your Price Quantity
M0211S 40,000 units/ml 10,000 units $83.00 $83.00
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  • EcoRI Methyltransferase modifies the internal adenine residue (N6) of the sequence GAATTC.

    Product Source

    An E. coli. strain that carries the cloned EcoRI modification gene from Escherichia coli RY13 (R. N. Yoshimori).
    This product is related to the following categories:
    Methyltransferases for Epigenetics,
    Base Modifying Enzymes

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by EcoRI restriction endonuclease

      Reaction Conditions

      1X rCutSmart™ Buffer
      Supplement with 80 µM S-adenosylmethionine (SAM)
      Incubate at 37°C

      1X rCutSmart™ Buffer
      50 mM Potassium Acetate
      20 mM Tris-acetate
      10 mM Magnesium Acetate
      100 µg/ml Recombinant Albumin
      (pH 7.9 @ 25°C)

      Control Protection Assay

      rCutSmart Buffer is incubated with 1 µg of λ DNA in 10 µl 1X EcoRI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by EcoRI Methyltransferase is determined by the addition of 40 µl rCutSmart Buffer supplemented with 10 mM MgCl2 and 5 units of EcoRI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on an agarose gel.

      Storage Buffer

      100 mM KPO4
      200 mM NaCl
      10 mM β-ME
      0.1 mM EDTA
      200 µg/ml BSA
      50% Glycerol
      pH 7.4 @ 25°C

      Heat Inactivation

      65°C for 20 minutes

      Molecular Weight

      Theoretical: 37911 daltons

    • Related Products

      Materials Sold Separately

    • Product Notes

      1. EcoRI Methyltransferase is inhibited by MgCl2. Only 50% activity is retained at a concentration of 4 mM MgCl2.

    • References

      1. Hoffman, J.L. (1986). biochemistry. 25, 4444-4449.

  • Protocols, Manuals & Usage

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      EcoRI Methyltransferase is now supplied with rCutSmart Buffer (12/1/2021)
      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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