PURExpress
PURExpress® (NEB #E6800) is a reconstituted protein synthesis system based on the PUREsystem™ (Shimizu et al., 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli.
- Defined system with all His-tagged proteins for coupled transcription/translation; Ribosome is not His-tagged
- T7 RNA Polymerase drives in vitro transcription
- Minimal nuclease and protease activity for stability of synthesized protein and encoding target
- Templates can be either plasmid DNA, linear DNA or mRNA
- Protein of interest can be synthesized and visualized in a few hours
- Synthesizes various target peptides and proteins
- Synthesized protein can be co-translationally radiolabeled or fluorescently labeled
- Protein can be reverse-purified or subject to direct functional analysis
- Applications include high throughput screening/directed evolution, synthetic biology, toxic or difficult to express protein synthesis, studies on protein folding, activity and protein-protein interactions
- Due to reconstituted nature, several kits are offered where translation factors or macromolecules have been omitted to facilitate specific studies; PURExpress® ∆ (aa, tRNA) Kit (NEB #E6840), PURExpress® ∆ RF123 Kit (NEB #E6850), PURExpress® ΔRibosome Kit (NEB #E3313)
- Compatible with the PURExpress Disulfide Bond Enhancer (NEB #E6820)
Choose Type:
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
- Protein Expression & Purification Brochure
- Protein Expression and Purification Selection Chart
- PURExpress® vs. NEBExpress® Application Chart
Feature Articles
Brochures
Selection Tools
- His-Tagged Translation Factors
- Initiation Factors (IF1, IF2, IF3)
- Elongation Factors (EF-Tu, EF-Ts, EF-G)
- Release Factors (RF1, RF2, RF3)
- Ribosome Recycling Factor
- 20 Aminoacyl tRNA synthetases
- Methionyl tRNA formyltransferase
- E. coli Ribosomes
- E. coli tRNAs
- Energy Regeneration System
- NTPs, Amino Acids, Salts, Buffer
In addition, recombinant T7 RNA polymerase is used to couple transcription to translation. The PURE system represents an important step towards a totally defined in vitro transcription/translation system, thus avoiding the “black box” nature of the cell extract-based systems.
PUREXPRESS® is a registered trademark of New England Biolabs, Inc..
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
