
Protein Expression
Choose Type:
- Cell-Free Protein Expression
- NEBExpress® Cell-free E. coli Protein Synthesis System
- PURExpress
- Protein Expression in Yeast
- Expression of Difficult Proteins
- Disulfide-bonded Protein Expression
- Membrane Protein Expression
- Toxic Protein Expression
- Target Protein Insolubility
- Protein Expression in E. Coli
- T7 Expression
- Non-T7 Expression
- Protein Expression Using BL21(DE3) (C2527)
- Protein Expression with T7 Express strains
- Expression Using SHuffle®
- Protein Synthesis Reaction using PURExpress (E6800)
- Western Analysis (E8023)
- Transformation Protocol (C2530)
- Protocol for Protein Expression Using BL21 (C2530)
- 5 Minute Transformation Protocol (C2530)
- Expression Using SHuffle (C3027)
- Use SNAP-Capture Pull Down Resin (S9144)
- Electroporation Protocol (C2986)
- Expression Using SHuffle (C3026)
- E. coli Lemo21(DE3)
- Co-expression of Multiple Proteins in Kluyveromyces lactis
- NEBExpress® Cell-free E. coli Protein Synthesis System
- Protein Expression with T7 Express Strains
- Transformation of SHuffle® Competent Cell Strains
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
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Over 40 years in protein expression and purification – a historical perspective
This article provides an overview of the advances in protein expression and purification methodology over the past 40 years.
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
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The Next Generation of Reagents for Sample Preparation
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Why Choose the K. lactis Protein Expression Kit?
Review the advantages of the K. lactis Protein Expression Kit for rapid, high yield protein expression in yeast.
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- DNA Sequences and Maps Tool
- Competent Cell Product Comparison
- IMPACT™ Vectors and Applications
- Protein Expression and Purification Selection Chart
- An E.coli lysate-based system for in vitro Protein Synthesis
Feature Articles
Brochures
Web Tools
Selection Tools
Posters
- Sakhtah, H., Behler, J., Ali-Reynolds, A., Causey, T.B., Vainauskas, S., Taron, C.H. 2019. A novel regulated hybrid promoter that permits autoinduction of heterologous protein expression in Kluyveromyces lactos Appl. Environ. Microbiol.. , PubMedID: 31053583, DOI:
- Reuter, W.H., Masuch, T., Ke, N., Lenon, M., Radzinski, M., Van Loi, V., Ren, G., Riggs, P., Antelmann, H., Reichmann, D., Leichert, L.I., Berkmen, M 2019. Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote Redox Biol. 26, PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
- Chuzel, L., Ganatra, M.B., Schermerhorn, K.M., Gardner, A.F., Anton, B.P., Taron, C.H. 2017. Complete genome sequence of Kluyveromyces lactis strain GG799, a common yeast host for heterologous protein expression Genome Announcements. 5(30), PubMedID: 28751387, DOI:
- Mauris, J.and Evans, T.C., Jr. 2010. A human PMS2 homologue from Aquifex aeolicus stimulates an ATP-dependent DNA helicase. J Biol.Chem. 285(15), PubMedID: 20129926, DOI:
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