Experts in protein expression and purification
Protein expression can be a very complex, multi-factorial process. Each protein requires a specific intracellular environment to correctly achieve its secondary and tertiary structures. Proteins may also require post-translational modifications or insertion into a cellular membrane for proper function. Other proteins, once expressed, may be toxic to the host. Thus, no single solution exists for the successful production of all recombinant proteins, and a broad range of expression tools is needed to ensure the successful expression of your target protein.
Our NEBExpress™ portfolio of products includes solutions for expression and purification, and is supported by access to scientists with over 45 years of experience in developing and using recombinant protein technologies in E. coli. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.
Generate analytical amounts of protein with our cell-free expression systems
- The PURExpress® In Vitro Protein Synthesis Kit our novel cell-free transcription/translation system, enables protein expression in approximately two hours and is ideal for high throughput technologies
- NEBExpress Cell-free E. coli Protein Synthesis System, our novel E. coli cell-extract based transcription/translation system, is designed to synthesize proteins under the control of T7 RNA Polymerase at high yields for a wide variety of proteins
Generate and purify high yields of recombinant proteins
- The NEBExpress MBP Fusion and Purification System utilizes a pMAL vector and the malE gene for the expression of MBP-fusion proteins, which can be isolated by a two-step affinity purification
- The IMPACT™ Kit utilizes engineered protein splicing elements (inteins) to purify recombinant proteins in a single step
- For analysis, try our protein standards, which are available unstained, prestained, or with two colors for easy identification
Express a variety of proteins with our competent cells
- Our popular BL21 and BL21(DE3) Competent E. coli strains are available for routine expression
- Lemo21(DE3) Competent E. coli offers tunable T7 expression for difficult targets
- For expression of His-tagged proteins, we offer NiCo21(DE3) Competent E. coli
- SHuffle® strains are available for the expression of proteins with multiple disulfide bonds
Purify tagged proteins with our magnetic beads, columns and resins
- Nickel spin columns, magnetic beads and resin enable rapid purification of His-tagged proteins
- Amylose resins for purification of MBP-tagged proteins are available in a variety formats (standard, high flow and magnetic)
- Chitin resin allows for rapid purification of CBD-tagged proteins
- Remove affinity tags following your purification with TEV Protease
One or more of these products are covered by patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at gbd@neb.com. The use of these products may require you to obtain additional third party intellectual property rights for certain applications.
Featured Products:
NEBExpress® Salt Active Nuclease
Patent-pending endonuclease that non-specifically cleaves and degrades DNA and RNA in AAV workflows, with higher activity than comparable nucleases and optimal activity in high salt.
Learn more with these protein expression-related tools:
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Protein Expression and Purification Brochure Learn more about NEB’s products for protein expression and purification, including expression and purification kits, ladders, competent cells and more. |
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Competent Cells Brochure Find information on our competent cells available for the expression of a variety of proteins. |
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Purification Beads, Columns and Resins Brochure Learn more about NEB's portfolio of products for protein purification. |
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Protein Standard Magnet Request a copy of this popular reference tool. |
Learn more from featured application notes: |
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Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads |
