The entire complement of proteins, protein-protein interactions and post-translational modifications within an organism is known as its proteome; the moniker being a blend of protein and genome. Since protein modifications, such as phosphorylation, acetylation, glycosylation and methylation, are state-dependent, the proteome is constantly changing in response to cellular cues. Proteome-focused studies, then, must specify the conditions under which the proteome was analyzed. Proteomics is the study of the proteome. Like genomics, proteomics is a branch of bioanalysis that provides valuable data about a cell. Unlike genomics, in which data can be gathered from any cell of an organism, proteomics relies on cell specific, temporal and environment specific readouts.
Modern proteomics benefits from the ability to assess the modification state of proteins directly, using a combination of techniques such as mass spectrometry, western blotting and enrichment. The proteome of a cell is of much greater complexity than the genome.
- Which residues does Endoproteinase AspN cut?
- Are there any amino acid residues that inhibit or reduce the efficiency of digestion of glutamate residues in a peptide sequence with Endoproteinase GluC? The site I want to digest has a glutamate residue followed by a proline residue and some enzymes are inhibited by the presence of a proline after the hydrolysis site.
- I am using Trypsin and am wondering about specificity. Does it cut at additional sites when in high concentration?
- Is there a simple way to remove Trypsin after protein cleavage?
- How does one do a Trypsin in-gel digest?
- I have a very low concentration of protein and would prefer not to denature as a separate step with buffer exchange before digestion. What denaturants can he use in the Trypsin reaction itself?
- What is the Proteinase K activity in commonly used buffers?
- Cleavage of the Fusion Protein Generated Using The pMAL Protein Fusion and Purification System (E8200)
- Cloning a PCR Fragment Into a pMAL Expression Vector (E8200)
- O-Glycosidase Application Note 1 (P0733)
- O-Glycosidase (P0733)
- Purification of a Fusion Protein generated by The pMAL Protein Fusion and Purification System (E8200)
- Reaction Protocols for Protein Deglycosylation Mix (P6039)
- Separating the Protein of Interest from MBP after Protease Cleavage Using The pMAL Protein Fusion and Purification System (E8200)
- Western Transfer Protocol for Anti-MBP Monoclonal Antibody
- Endo-α-N-Acetylgalactosaminidase Application Note 1
- Quick Start Guide (E8200)
- Removal of terminal N-acetylglucosamine from the biantennary N-linked sugars of IgG
- Typical Reaction Conditions for α2-3,6,8 Neuraminidase (P0720)
- Endo H/Endo Hf Protocol
- Typical Reaction Conditions (P0732)
- Typical Reaction Conditions for α2-3 Neuraminidase (P0728)
- Typical Reaction Conditions for β1-4 Galactosidase (P0730)
- Reaction Conditions for Remove-iT® PNGase F (P0706)
- Reaction Conditions for Endo S (P0741)
- Endo S Removal Magnetic Chitin Bead Protocol (P0741)
- Remove-iT® PNGase F Magnetic Chitin Bead Protocol (P0706)
- Reaction Conditions for Endo D (P0742)
- Endo D Removal Magnetic Chitin Bead Protocol (P0742)
- RNase B Deglycosylation Protocol (P7817)
- PNGase F Protocol
- Typical Reaction Conditions for α2-3,6,8,9 Neuraminidase A (P0722)
- Typical Reaction Conditions for α1-3, 4, 6 Galactosidase (P0747)
- Typical Reaction Conditions for α1-2, 3, 4, 6 Fucosidase (P0748)
- Typical Reaction Conditions for α1-3, 4 Fucosidase (P0769)
- Reaction Conditions for IdeZ Protease (IgG-specific) (P0770)
- Reaction conditions for Simultaneous Digestion of IgG with IdeZ Protease (IgG-specific) and PNGase F (fragmentation and deglycosylation) (P0770)
- Glycoproteomics: Buffer Exchange Protocols (P0711)
- Rapid PNGase F (non-reducing format) (P0711) Reaction Protocol
- Rapid PNGase F (non-reducing format) (P0711) SDS-PAGE Protocol
- Typical Reaction Conditions for Endo F3 Protocol (P0771)
- Reaction Conditions for PNGase A (P0707)
- In-gel Digestion Protocol for Endoproteinase LysC (P8109)
- Typical Reaction Conditions for Endoproteinase LysC
- Endo F2 Reaction Protocol (P0772)
- Removal of Endo F2 by Magnetic Beads (P0772)
- a1-2,4,6 Fucosidase O Digestion of Released Labeled Glycans Protocol
- Leith, E.M., O'Dell, W.B., Ke, N., McClung, C., Berkmen, M., Bergonzo, C., Brinson, R.G., Kelman, Z 2019. Characterization of the internal translation initiation region in monoclonal antibodies expressed in Escherichia coli J Biol Chem. 294(48), PubMedID: 31604819, DOI: 10.1074/jbc.RA119.011008
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