The DNA repair step is easily integrated into sequencing workflows for any platform, upstream of library preparation, and treatment results in both increased library yields and increased library quality.
Table 1: Types of FFPE DNA damage and their ability to be repaired by the NEBNext FFPE DNA Repair Mix
|FFPE DAMAGE TYPE||REPAIRED BY THE FFPE DNA ENZYME REPAIR MIX?|
|Deamination of cytosine to uracil||Yes|
|Nicks and gaps||Yes|
|Blocked 3´ ends||Yes|
- Protocol for use with NEBNext Ultra DNA Library Prep Kit for Illumina (E7370)
- Protocol for use with NEBNext FFPE DNA Repair Mix (M6630) and NEBNext DNA Library Prep Master Mix Set for Illumina (E6040)
- NEBNext FFPE DNA Repair Mix (M6630) - Protocol for use with Other User-supplied Library Construction Reagents
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Behind the paper: DNA damage is a pervasive cause of sequencing errors, directly confounding variant identification
NEB researchers published a paper in Science highlighting DNA damage as a prevalent source of errors in public cancer databases. Learn about how addressing this damage could improve the detection of low-frequency disease variants.