- Protocol for Dephosphorylation of 5'-ends of DNA using CIP (NEB #M0290)
- Protocol for Dephosphorylation of 5´-ends of DNA using Antarctic Phosphatase (NEB #M0289)
- Protocol for Dephosphorylation of 5´-ends of DNA using rSAP (M0371)
- Protocol for Dephosphorylation of 5´-ends of DNA using Quick Dephosphorylation Kit (M0508)
- Enzymatic PCR Cleanup Protocol
- Molecular Cloning Technical Guide
- DNA Ligase Selection Chart
- Phosphatase Selection Chart
- Troubleshooting Guide for End Modification
- Troubleshooting Tips for Ligation Reactions
- Tips for Maximizing Ligation Efficiencies
- Traditional Cloning Quick Guide
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Dephosphorylation is the process by which phosphate groups are removed from a molecule by a phosphatase. Removal of phosphate groups from a DNA fragment can prevent ligation. Learn more about dephosphorylation and phosphatases.