Enzymatic PCR Cleanup Protocol (NEB #M0371)

Enzymatic PCR Cleanup Protocol 

1. Set-up the reaction as follows:



COMPONENTS	Reaction Volumes
PCR Product	5 µl
Exonuclease I (NEB #M0293)	0.5 µl (10 units)
Shrimp Alkaline Phosphatase (rSAP)* (NEB #M0371)	1 µl (1 unit)

*[Note that the same volume of Quick CIP (NEB #M0525) can be substituted for rSAP.]

2. Incubate the mix at the 37°C for 15 minutes.
3. Inactivate enzymes at 80°C for 15 minutes.

PCR products are ready for downstream application.

Interested in a 5-minute protocol? Try out the Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050)