Cellular Analysis

Cellular Analysis

Studies of protein properties, expression, transport, degradation and their interactions with other cellular systems are integral to biomedical research, drug discovery and developmental biology. Eukaryotic proteins function in signaling pathways, metabolism, structure, adhesion, cell movement, active and passive transport, DNA repair, viral disease mechanisms, the immune system, fertilization, differentiation, epigenetics, cancer and the cell division cycle.

For efficient analysis of cellular protein interactions and expression, target genes are often engineered into reporter systems, and then expressed in cells as recombinant fusion proteins. Reporter gene characteristics are chosen to enable downstream in vitro or in vivo method applications. This commonly used and widely successful strategy can facilitate applications such as flow cytometry, cell sorting, in vitro or in vivo imaging, proteomic microarray, cell-based microarray, SDS-PAGE gel electrophoresis, Western blot, quantitative affinity purification followed by mass spectrometry (q-AP-MS), pulse-chase and receptor internalization assays. In particular, improved reporter systems in conjunction with optical imaging technical advances are pushing the frontiers of fluorescence imaging applications.

SNAP-tag® is a registered trademark of New England Biolabs, Inc.
  1. Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging

    Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.

  2. SNAP-tag Overview Tutorial

    View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.

Cellular Analysis includes these areas of focus:

Reporter Systems
Cypridina Luciferase
Gaussia Luciferase
Transfection
RNA Transfection
Protein Transfection
Cell Imaging
SNAP Cell
SNAP Companion
SNAP Surface
CLIP Cell
CLIP Companion
CLIP Surface
ACP/MCP Surface
ACP/MCP Companion
In vivo Imaging
Protein Localization
Pulse Chase
Receptor Internalization

FAQs for Cellular Analysis

Protocols for Cellular Analysis

    Publications related to Cellular Analysis:

  1. Domoszlai T, Martincuks A, Fahrenkamp D, Schmitz-Van de Leur H, Küster A, Müller-Newen G (2014). Consequences of the disease-related L78R mutation for dimerization and activity of STAT3 J Cell Sci. 127(Pt 9), 1899-910. PubMedID: 24569879, DOI: 10.1242/jcs.137422