Studies of protein properties, expression, transport, degradation and their interactions with other cellular systems are integral to biomedical research, drug discovery and developmental biology. Eukaryotic proteins function in signaling pathways, metabolism, structure, adhesion, cell movement, active and passive transport, DNA repair, viral disease mechanisms, the immune system, fertilization, differentiation, epigenetics, cancer and the cell division cycle.
For efficient analysis of cellular protein interactions and expression, target genes are often engineered into reporter systems, and then expressed in cells as recombinant fusion proteins. Reporter gene characteristics are chosen to enable downstream in vitroor in vivo method applications. This commonly used and widely successful strategy can facilitate applications such as flow cytometry, cell sorting, in vitro or in vivo imaging, proteomic microarray, cell-based microarray, SDS-PAGE gel electrophoresis, Western blot, quantitative affinity purification followed by mass spectrometry (q-AP-MS), pulse-chase and receptor internalization assays. In particular, improved reporter systems in conjunction with optical imaging technical advances are pushing the frontiers of fluorescence imaging applications.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
Cellular Analysis includes these areas of focus:
- RNA Transfection
- Protein Transfection
- Protein Localization
- Pulse Chase
- Receptor Internalization
- In vivo Imaging
- Cell Imaging
- SNAP Companion
- SNAP Surface
- CLIP Cell
- CLIP Companion
- CLIP Surface
- ACP/MCP Surface
- SNAP Cell
- ACP/MCP Companion
- Reporter Systems
- Cypridina Luciferase
- Gaussia Luciferase
FAQs for Cellular Analysis
Protocols for Cellular Analysis
- Cellular Labeling (S9101)
- Cellular Labeling (S9103)
- Cellular Labeling (S9104)
- Labeling of Proteins in vitro (S9101)
- Labeling of Proteins in vitro (S9103)
- Labeling of Proteins in vitro (S9104)
- View the video "Fluorescent Labeling of COS-7 Expressing SNAP-tag Fusion Proteins for Live Cell Imaging" in the Journal of Visualized Experiments (JoVE)
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- SNAPf based pulse labeling for analysis of protein turnover in living cells
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Simultaneous Labeling of Two Proteins in Live Cells
- Ultrasensitive Secreted Luciferases and their use in a Dual Reporter System
Cellular Imaging and Analysis Brochure
The Cellular Imaging and Analysis brochure provides information on the labeling technologies offered by NEB for studying the function and localization of proteins in cells.
- Gaussia and Cypridina Luciferases - Ultrasensitive secreted reporters and their use in dual assays
- SNAP-tag® Technologies: Novel Tools to Study Protein Function
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
Other Tools & Resources
- Domoszlai T, Martincuks A, Fahrenkamp D, Schmitz-Van de Leur H, Küster A, Müller-Newen G 2014. Consequences of the disease-related L78R mutation for dimerization and activity of STAT3 J Cell Sci. 127(Pt 9), PubMedID: 24569879, DOI: 10.1242/jcs.137422
Publications related to Cellular Analysis
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at email@example.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.