Studies of protein properties, expression, transport, degradation and their interactions with other cellular systems are integral to biomedical research, drug discovery and developmental biology. Eukaryotic proteins function in signaling pathways, metabolism, structure, adhesion, cell movement, active and passive transport, DNA repair, viral disease mechanisms, the immune system, fertilization, differentiation, epigenetics, cancer and the cell division cycle.
For efficient analysis of cellular protein interactions and expression, target genes are often engineered into reporter systems, and then expressed in cells as recombinant fusion proteins. Reporter gene characteristics are chosen to enable downstream in vitroor in vivo method applications. This commonly used and widely successful strategy can facilitate applications such as flow cytometry, cell sorting, in vitro or in vivo imaging, proteomic microarray, cell-based microarray, SDS-PAGE gel electrophoresis, Western blot, quantitative affinity purification followed by mass spectrometry (q-AP-MS), pulse-chase and receptor internalization assays. In particular, improved reporter systems in conjunction with optical imaging technical advances are pushing the frontiers of fluorescence imaging applications.
New England Biolabs offers an innovative technology for studying the function and localization of proteins in living and fixed cells. Covalent protein labeling offers simplicity and versatility to the imaging of mammalian proteins in live cells, as well as the ability to capture proteins in vitro. A single genetic construct generates a fusion protein which, when covalently attached to a variety of fluorophores, biotin or beads, provides a powerful tool for studying protein dynamics. In the first system, the protein is labeled by a self-labeling fusion protein; SNAP-tag® or CLIP-tag™.
SNAP-tag® is a registered trademark of New England Biolabs, Inc.
- Labeling and Imaging of Cell Surface Receptors Mediated by SNAP-tag®
- Labeling of Escherichia coli Expressed SNAP-tag® Fusion Proteins
- Simultaneous Fluorescent Labeling of Proteins in Living Cells
- Simultaneous Labeling of Two Proteins in Live Cells
- SNAPf based pulse labeling for analysis of protein turnover in living cells
- Ultrasensitive Secreted Luciferases and their use in a Dual Reporter System
Gaussia and Cypridina Luciferases - Ultrasensitive secreted reporters and their use in dual assays
SNAP-tag® Technologies: Tools to Study Protein Function
Read about the NEB’s set of protein tools for the specific labeling (SNAP-, CLIP-, ACP- and MCP-tags) of fusion proteins.
- Cellular Imaging & Analysis Brochure
- Comparison of SNAP-tag®/CLIP-tag™ Technologies to GFP
- Labeling with SNAP-tag® Technology Troubleshooting Guide
- Genome-wide profiling of nuclease protected domains reveals physical properties of chromatin (2019)
- Improving Transcriptome Profiling for Single Cell and Low Input RNA (2019)
- In Vitro Reconstitution of Thermococcus Species 9°N Okazaki Fragment Maturation (2015)
- One Ring To Bind Them All: Is HemeBiosynthesis A Factor In Wolbachia-Filarial Nematode Endosymbiosis? (2015)
- Reuter, W.H., Masuch, T., Ke, N., Lenon, M., Radzinski, M., Van Loi, V., Ren, G., Riggs, P., Antelmann, H., Reichmann, D., Leichert, L.I., Berkmen, M 2019. Utilizing redox-sensitive GFP fusions to detect in vivo redox changes in a genetically engineered prokaryote Redox Biol. 26, PubMedID: 31450103, DOI: 10.1016/j.redox.2019.101280
- Maffei, M., Morelli, C., Graham, E., Patriarca, S., Donzelli, L., Doleschall, B., de Castro, Reis, F., Nocchi, L., Chadick, C.H., Reymond, L., Correa, I.R., Jr., Johnsson, K., Hackett, J.A., Heppenstall, P.A 2019. A ligand based system for receptor specific delivery of proteins Sci Rep. 9(1), PubMedID: 31844114, DOI: 10.1038/s41598-019-55797-1
- Carpinone, E.M., Li, Z., Mills, M.K., Foltz, C., Brannon, E.R., Carlow, C.K.S., Starai, V.J. 2018. Identification of putative effectors of the Type IV secretion system from the Wolbachia endosymbiont of Brugia malayi PLoS One. 13 (9), PubMedID: 30261054, DOI: 10.1371/journal.pone.0204736
- Ke, N., Landgraf, D., Paulsson, J. and Berkmen, M. 2016. Visualization of Periplasmic and Cytoplasmic Proteins with a Self-Labeling Protein Tag. J Bacteriol. Jan 19;198(7), PubMedID: 26787765, DOI:
- Domoszlai T, Martincuks A, Fahrenkamp D, Schmitz-Van de Leur H, Küster A, Müller-Newen G 2014. Consequences of the disease-related L78R mutation for dimerization and activity of STAT3 J Cell Sci. 127(Pt 9), PubMedID: 24569879, DOI: 10.1242/jcs.137422
- Johnson K. 2008. SNAP-tag Technologies: Novel tools to study protein function NEB Expressions . 3.3 , PubMedID: , DOI:
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Watch as Chris Provost, of New England Biolabs, performs fluorescent imaging of live COS-7 cells expressing SNAP-tag® fusion proteins.
View an interactive tutorial explaining the mechanism of our SNAP-tag® technologies and reagents available for researchers wishing to study the function and localization of proteins in live or fixed cells.