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NEBNext® High Input Poly(A) mRNA Isolation Module
Isolate intact poly(A)+ RNA from high inputs (5-50 µg) of previously isolated total RNA, using oligo d(T)25-coupled paramagnetic beads. Intact mRNA can be obtained in approximately one hour, and eluted in small volumes.
Ph.D.™-C7C Phage Display Peptide Library Kit v2
The Ph.D.-C7C Phage Display Peptide Library is based on a combinatorial library of random disulfide looped peptides fused to the N-terminus of a minor coat protein (pIII) of M13 phage. The Ph.D.-C7C™ Kit v2 contains the Ph.D.-C7C Phage Display Peptide Library, a DYKDDDDK Mouse monoclonal antibody and Protein G Magnetic Beads for a panning control experiment, and enough -96gIII sequencing primer for >50 sequencing reactions.
phi29-XT RCA Kit
This kit contains the components for optimized RCA: phi29-XT DNA Polymerase engineered for improved performance, exo-resistant random primers, and dNTPs.
This new enzyme mix for DNA fragmentation has been designed for use with challenging samples, including FFPE DNA and methylated DNA. NEBNext UltraShear maintains methylation marks on DNA.
NEBNext® FFPE DNA Library Prep Kit
This kit is designed for NGS library prep of pre-sheared FFPE DNA and includes reagents for repair and optimized library prep.
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 4)
The fourth set of Unique Dual Index UMI Adaptors for improved SNV detectiPCRon or PCR-free workflows.
NEBNext Ultrashear™ FFPE DNA Library Prep Kit
This kit for FFPE DNA library prep combines specialized enzymatic FFPE DNA fragmentation and repair, with optimized reagents and protocols to provide superior performance with this challenging sample type.
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 3)
The third set of Unique Dual Index UMI Adaptors for improved SNV detection or PCR-free workflows.
NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA Set 2)
The second set of Unique Dual Index UMI Adaptors for improved SNV detection or PCR-free workflows.
EnGen® Seq1 Cas9
EnGen Seq1 Cas9 from Streptococcus equinus is an RNA-guided DNA endonuclease that catalyzes site-specific cleavage of double-stranded DNA (dsDNA).
Thermostable USER® III Enzyme
Thermostable USER III Enzyme generates a single nucleotide gap at the location of a uracil residue. It is active between 50-75°C.
NEBExpress® T4 Lysozyme
NEBExpress® T4 Lysozyme is a recombinant murein hydrolase that breaks down the bacterial cell wall by hydrolyzing the -1,4 linkage between N-acetylmuramic acid and N-acetylglucosamine in the peptidoglycan of prokaryotic cells (gram-negative and some gram-positive bacteria). It can be used to extract soluble proteins, membrane proteins, DNA, RNA, or metabolites.
Authenticase is a mixture of structure-specific nucleases capable of recognizing and cleaving outside mismatch and indel regions from 1-10 bp on double-stranded DNA. The formulation has limited non-specific activity on homoduplex regions of DNA.
EnGen® Spy Cas9 HF1
EnGen® Spy Cas9 HF1 is a high-fidelity, quadruple substitution (N497A/R661A/Q695A/Q926A) variant of EnGen® Spy Cas9 NLS from Streptococcus pyogenes with reduced non-specific DNA cleavage.