Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

ApaI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

Please also check these additional information about:
RE Performance Chart | Double Digestion | Time-Saver Enzymes

Enzyme Optimal Temperature % Activity at 37 °C
AbaSI 25°C n/a
ApaI 25°C 100
ApeKI 75°C 10
ApoI§ 50°C 50
BaeI 25°C 20
BclI 50°C 50
BfuAI 50°C 50
BsaBI 60°C 20
BsaJI 60°C 20
BsaWI 60°C 20
BsiEI 60°C 30
BsiHKAI 65°C 5
BsiWI§ 55°C 50
BslI 55°C 30
BsmAI 55°C 50
BsmBI 55°C 20
BsmFI 65°C 50
BsmI 65°C 20
BspCNI 25°C 75
BspQI 50°C 10
BsrDI 65°C 30
BsrI 65°C 20
BssHII 50°C 75
BstAPI 60°C 10
BstBI 65°C 10
BstEII § 60°C 50
BstNI 60°C 30
BstUI 60°C 20
BstYI 60°C 30
BtgZI 60°C 75
BtsαI 55°C 75
BtsCI 50°C 50
BtsIMutI 55°C n/a
CviAII 25°C 20
CviQI 25°C 10
FatI 55°C 20
FauI 55°C 20
MwoI 60°C 10
Nb.BsmI 65°C 25
Nb.BsrDI 65°C 75
Nt.BspQI 50°C 80
Nt.BstNBI 55°C 10
PI-PspI 65°C 5
PspGI 75°C 10
SfiI 50°C 10
SmaI 25°C 50
SmlI 55°C 10
SwaI 25°C 50
TaqαI 65°C 10
TfiI 65°C 10
TseI 65°C 20
Tsp45I 65°C 10
TspMI 75°C 20
TspRI 65°C 10
Tth111I 65°C 10

§ An HF version of this enzyme is available.