Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

ApaI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

Please also check these additional information about:
RE Performance Chart | Double Digestion | Time-Saver Enzymes

EnzymeOptimal Temperature% Activity at 37 °C
AbaSI25°Cn/a
ApaI25°C100
ApeKI75°C10
ApoI §50°C50
BaeI25°C20
BclI50°C50
BfuAI50°C50
BsaBI60°C20
BsaJI60°C20
BsaWI60°C20
BsiEI60°C30
BsiHKAI65°C5
BsiWI §55°C50
BslI55°C30
BsmAI55°C50
BsmBI55°C20
BsmFI65°C50
BsmI65°C20
BspCNI25°C75
BspQI50°C10
BsrDI65°C30
BsrI65°C20
BssHII50°C75
BssKI60°C10
BstAPI60°C10
BstBI65°C10
BstEII  §60°C50
BstNI60°C30
BstUI60°C20
BstYI60°C30
BtgZI60°C75
BtsαI55°C75
BtsCI50°C50
BtsI55°C75
BtsIMutI55°Cn/a
CviAII25°C20
CviQI25°C10
FatI55°C20
FauI55°C20
MwoI60°C10
Nb.BsmI65°C25
Nb.BsrDI65°C20
Nt.BspQI50°C80
Nt.BstNBI55°C10
PhoI75°C0
PI-PspI65°C5
PspGI75°C10
SfiI50°C10
SmaI25°C50
SmlI55°C10
SwaI25°C50
TaqαI65°C10
TfiI65°C10
TliI75°C10
TseI65°C20
Tsp45I65°C10
Tsp509I65°C10
TspMI75°C20
TspRI65°C10
Tth111I65°C10

§ A HF version of this enzyme is available.