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  • Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

    Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

    ApaI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

    Please also check these additional information about:
    RE Performance Chart | Double Digestion | Time-Saver Enzymes

    EnzymeOptimal Temperature% Activity at 37 °C
    AbaSI25°C0
    ApaI25°C100
    ApeKI75°C10
    ApoI50°C50
    BaeI25°C20
    BclI50°C50
    BfuAI50°C50
    BsaBI60°C20
    BsaJI60°C20
    BsaWI60°C20
    BsiEI60°C30
    BsiHKAI65°C5
    BsiWI55°C50
    BslI55°C30
    BsmAI55°C50
    BsmBI55°C20
    BsmFI65°C50
    BsmI65°C20
    BspCNI25°C75
    BspQI50°C10
    BsrDI65°C30
    BsrI65°C20
    BssHII50°C75
    BssKI60°C10
    BstAPI60°C10
    BstBI65°C10
    BstEII 60°C50
    BstNI60°C30
    BstUI60°C20
    BstYI60°C30
    BtgZI60°C75
    BtsCI50°C50
    BtsI55°C75
    BtsIMutI55°Cn/a
    CviAII25°C20
    CviQI25°C10
    FatI55°C20
    FauI55°C20
    MwoI60°C10
    Nb.BsmI65°C25
    Nb.BsrDI65°C20
    Nt.BspQI50°C80
    Nt.BstNBI55°C10
    PhoI75°C0
    PI-PspI65°C5
    PspGI75°C10
    SfiI50°C10
    SmaI25°C50
    SmlI55°C10
    SwaI25°C50
    TaqαI65°C10
    TfiI65°C10
    TliI75°C10
    TseI65°C20
    Tsp45I65°C10
    Tsp509I65°C10
    TspMI75°C20
    TspRI65°C10
    Tth111I65°C10