Activity at 37°C for Restriction Enzymes with Alternate Incubation Temperatures

Listed below is the percentage activity exhibited at 37°C for enzymes that have an optimal incubation temperature higher (thermophiles) or lower (25°C) than 37°C.

ApaI has 100% activity at 37°C, however the half-life of this enzyme at 37°C is only 30 minutes.

Please also check these additional information about:
RE Performance Chart | Double Digestion | Time-Saver Enzymes

EnzymeOptimal Temperature
AbaSI25°C
ApaI25°C
ApeKI75°C
ApoI §50°C
BaeI25°C
BclI50°C
BfuAI50°C
BsaBI60°C
BsaJI60°C
BsaWI60°C
BsiEI60°C
BsiHKAI65°C
BsiWI §55°C
BslI55°C
BsmAI55°C
BsmBI55°C
BsmFI65°C
BsmI65°C
BspCNI25°C
BspQI50°C
BsrDI65°C
BsrI65°C
BssHII50°C
BssKI60°C
BstAPI60°C
BstBI65°C
BstEII  §60°C
BstNI60°C
BstUI60°C
BstYI60°C
BtgZI60°C
BtsαI55°C
BtsCI50°C
BtsI55°C
BtsIMutI55°C
CviAII25°C
CviQI25°C
FatI55°C
FauI55°C
MwoI60°C
Nb.BsmI65°C
Nb.BsrDI65°C
Nt.BspQI50°C
Nt.BstNBI55°C
PhoI75°C
PI-PspI65°C
PspGI75°C
SfiI50°C
SmaI25°C
SmlI55°C
SwaI25°C
TaqαI65°C
TfiI65°C
TliI75°C
TseI65°C
Tsp45I65°C
Tsp509I65°C
TspMI75°C
TspRI65°C
Tth111I65°C

§ A HF version of this enzyme is available.