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International iGEM Support

Products are available only to officially registered high school and college/university iGEM teams. Delivery could take as long as 1-2 weeks depending on your location.

International iGEM teams: please note additional processing via our international partners is required for these products. Please contact your local supplier for further information and delivery schedules.

NEB reserves the right to limit the number of free products and the right to discontinue this program at any time. Competent E. coli are not available for shipment to some countries.

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Thank you for your inquiry. We regret to inform you that under current U.S. law, NEB is prohibited from conducting business with individuals and entities in your country of residence. As such, we are unable to respond to your request at this time.

Note: Shipments to metropolitan France only.

Team Information

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Title

First Name

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Please provide an institutional address, we are unable to ship to residences.

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To assist with correct delivery, please provide as much detail as possible when entering delivery address.

Requested Delivery Date

NEB Materials Selection

DNA Assembly Mixes (choose up to 1):

NEBridge® Golden Gate Assembly Kit (BsmBI-v2) (NEB #E1602S)NEBridge® Golden Gate Assembly Kit (BsaI-HF®v2) (NEB #E1601S)NEBuilder® HiFi DNA Assembly Master Mix (NEB #E2621S) NEBuilder® HiFi DNA Assembly Cloning Kit ** (NEB #E5520S)

DNA Purification Kits (choose up to 2):

Monarch® Spin Plasmid Miniprep Kit (NEB #T1110S)Monarch® Spin PCR & DNA Cleanup Kit (5 μg) (NEB #T1130S)Monarch® Spin DNA Gel Extraction Kit (NEB #T1120S)

Competent Cells (choose up to 1):

NEB 5-alpha Competent E. coli ** (NEB #C2987I - Not available if ordering #E5520S)NEB 10-beta Competent E. coli ** (NEB #C3019I - Not available if ordering #E5520S)NEB Stable Competent E. coli ** (NEB #C3040I - Not available if ordering #E5520S)

** These items must be used immediately or placed in -80°C storage. Please check here to indicate that you fulfill this requirement. Please note competent cells are included with NEBuilder HiFi DNA Assembly Cloning Kit. Competent cells are not available in all countries.

I Understand

Type IIS Restriction Enzymes (choose up to 2):

BbsI-HF® (NEB # R3539S)BsaI-HF®v2 (NEB #R3733S)BsmBI-v2 (NEB #R0739S) Esp3I (NEB #R0734S)PaqCI® (NEB #R0745S)SapI (NEB #R0569S)

DNA Purification (choose up to 2):

Monarch® Plasmid Miniprep Kit (NEB #T1010S)Monarch® DNA Gel Extraction Kit (NEB #T1020S)Monarch® PCR & DNA Cleanup Kit (5 μg) (NEB #T1030S)Monarch® Genomic DNA Purification Kit (NEB #T3010S)

DNA Ligase (choose up to 1):

T4 DNA Ligase (NEB #M0202S)Hi-T4™ DNA Ligase (NEB #M2622S)NEBridge® Ligase Master Mix (NEB #M1100S)

Other (choose up to 2):

Q5® High-Fidelity 2X Master Mix (NEB #M0492S) Quick-Load® Purple 1kb Plus DNA Ladder (NEB #N0550S)

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Videos

Introduction to NEBuilder® HiFi DNA Assembly

Find out how NEBuilder^® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over NEB Gibson Assembly^®.
Golden Gate Assembly Workflow

Find out how Golden Gate Assembly can be used to quickly join multiple DNA fragments.
Golden Gate Assembly Domestication Tutorial

This video discusses Domestication, or the removal of Type IIS cut sites naturally occurring in vector or insert sequences, as it relates to Golden Gate Assembly.

Videos

Introduction to NEBuilder® HiFi DNA Assembly

Golden Gate Assembly Workflow

Golden Gate Assembly Domestication Tutorial

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