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CLIP-Surface™ 547

CLIP-Surface™ 547 is a photostable red fluorescent substrate that can be used to label CLIP-tag™ fusion proteins on the surface of living cells or in vitro.

  • This is a cell impermeable substrate (BC-547) based on the Dyomics dye DY-547
  • It is suitable for use with standard TAMRA or Cy3 filter sets
  • It has an excitation maximum at 554 nm and emission maximum at 568 nm

Ordering Information

$name
  • 50 nmol
    $307.00
  • Product Information
    This package includes 50 nmol of CLIP-Surface 547 substrate, sufficient to make 10 ml of a 5 µM CLIP-tag fusion protein labeling solution.

    The CLIP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. CLIP-tag is a protein tag based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). CLIP-tag substrates are derivatives of benzylcytosine (BC). In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the reactive cysteine of CLIP-tag forming a stable thioether bond. Although CLIP-tag is based on the same protein as SNAP-tag®, the benzylcytosine substrates form a separate class of substrates, different from the benzylcytosine substrates form a separate class of substrates, different from the benzylguanine substrates recognized by SNAP-tag. CLIP-tag and SNAP-tag can be used for orthogonal and complementary labeling of two proteins simultaneously in the same cells.

    There are two steps to using this system: subcloning and expression of the protein of interest as a CLIP-tag fusion, and labeling of the fusion with the CLIP-tag substrate of choice. Expression of CLIP-tag fusion proteins is described in the documentation supplied with CLIP-tag plasmids. The labeling of the fusion proteins on the cell surface with the CLIP-tag substrate is described in this document.

    Figure 1:Live CHO-K1 cells transiently transfected with pCLIP-NK1R
    Figure 1:Live CHO-K1 cells transiently transfected with pCLIP-NK1R
    Cells were labeled with CLIP-Surface 547 (red) for 60 minutes and counterstained with Hoechst 33342 (blue).
    Figure 2: Excitation (dotted line) and emission (full line) spectra of CLIP-Surface 547 coupled to CLIP-tag in buffer at pH 7.5
    Figure 2: Excitation (dotted line) and emission (full line) spectra of CLIP-Surface 547 coupled to CLIP-tag in buffer at pH 7.5
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    Figure 3. Structure of CLIP-Surface 547 (MW 851.0 g/mol)
    Product Categories:
    CLIP-tag™ Substrates Products,
    Protein Labeling Products
    Applications:
    CLIP Surface,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
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