SNAP-Capture Pull-Down Resin
The SNAP-Capture Pull Down Resin is used to selectively capture and immobilize a SNAP-tag® fusion protein from solution using agarose beads. The SNAP-Capture Pull Down Resin is prepared by the coupling of SNAP-tag substrate benzylguanine with a highly cross-linked agarose (4%) with excellent physical characteristics.
- These beads have a high loading capacity for SNAP-tag fusion proteins
- They show very low non-specific absorption of proteins from a complex lysate
- They are especially suitable for pull down applications
Two ml of SNAP-Capture Pull Down Resin is sufficient to perform 25 pull down assays using 80 µl of resin suspension per assay.
The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.
There are two steps to the use of this system: sub cloning and expression of the protein of interest as a SNAP-tag fusion, and capture and immobilization of the fusion protein using SNAP-Capture Pull Down Resin.
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