SNAP-Cell® 647-SiR

SNAP-Cell® 647-SiR is a far-red fluorescent substrate that can be used to label SNAP-tag® fusion proteins inside living cells, on cell surfaces, or in vitro.

  • It is a cell-permeable substrate (also termed SiR-SNAP) based on 6-carboxy-tetramethylsiliconrhodamine
  • It is suitable for standard Cy5 filter sets
  • It has an excitation maximum at 645 nm and an emission maximum at 661 nm

Ordering Information

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  • 30 nmol
    $322.00
  • Product Information
    This package contains 30 nmol of SNAP-Cell 647-SiR substrate, sufficient to make 10 ml of a 3 μM SNAP-tag fusion protein labeling solution.

    The SNAP-tag protein labeling system enables the specific, covalent attachment of virtually any molecule to a protein of interest. The SNAP-tag is based on human O6-alkylguanine-DNA-alkyltransferase (hAGT). SNAP-tag substrates are fluorophores, biotin or beads conjugated to guanine or chloropyrimidine leaving groups via a benzyl linker. In the labeling reaction, the substituted benzyl group of the substrate is covalently attached to the SNAP-tag.

    There are two steps to using this system: subcloning and expression of the protein of interest as a SNAP-tag fusion, and labeling of the fusion with the SNAP-tag substrate of choice. Expression of SNAP-tag fusion proteins is described in the documentation supplied with SNAP-tag plasmids. The labeling of the fusion proteins with the SNAP-tag substrate is described below.

    Figure 1. Live NIH/3T3 cells expressing SNAP-tag fused to mitochondrial oxidase 8A (Cox8A-SNAPf)

    Figure 1. Live NIH/3T3 cells expressing SNAP-tag fused to mitochondrial oxidase 8A (Cox8A-SNAPf)

    Cells were labeled with SNAP-Cell 647-SiR (red) for 30 minutes at 37°C, 5% CO2.
    Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell 647-SiR after coupling to SNAP-tag in buffer at pH 7.5Figure 2: Excitation (dotted line) and emission spectra of SNAP-Cell 647-SiR after coupling to SNAP-tag in buffer at pH 7.5

    Figure 3: Structure of SNAP-Cell 647-SiR (MW 724.9 g/mol)
    Figure 3: Structure of SNAP-Cell 647-SiR (MW 724.9 g/mol)
    Product Categories:
    SNAP-tag Substrates,
    Protein Labeling Products
    Applications:
    SNAP Cell,
    In vivo Imaging,
    Pulse Chase
    ,
    Receptor Internalization
    ,
    Protein Localization
    ,
    Protein Labeling (SNAP/CLIP)
    • Properties and Usage
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