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Random Primer Mix

  • Random priming is widely used in first strand cDNA synthesis.
  • Random Primer Mix is a ready-to-use optimized mixture of hexamers and anchored-dT primer (dT23VN).
  • A mixture of hexamers and anchored-dT primer provides even and consistent coverage of the RNA template population across a wide range of RNA template concentration. In contrast, traditional random priming by hexamer has poor coverage of 3´ end of RNA templates (1,2,3).

Ordering Information

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  • 60 μM
    100 μl
    $95.00
  • Product Information
    We recommend using Random Primer Mix for reverse transcription of the following RNA templates:

    1. RNA without poly(A) tail such as ribosomal RNAs.
    2. RNA with strong secondary structures.
    3. Partially degraded RNA samples.
    4. Target regions at 5´ end of a long messenger RNA transcript.
    Figure 1: 
    Figure 1
    Various amounts of human spleen total RNA (2 μg to 20 pg) were reverse transcribed using ProtoScript First Strand cDNA Synthesis Master Mix Kit (NEB #E6300 ) in the presence of 6 μM Random Primer Mix (panel A) or 3.5 μM random hexamers (panel B). About 1/10th of the cDNA products were then analyzed in duplicate qPCR reactions using a pair of primers specific for the beta actin gene with DyNAmo™ FLASH SYBR® Green qPCR Master Mix on a Bio-Rad IQ5® system.
    Product Categories:
    Reverse Transcriptases & RT-PCR
    Applications:
    RT-PCR & cDNA Synthesis,
    RT-qPCR, RT-PCR and cDNA Synthesis
    • Properties and Usage

      Storage Temperature

      -20°C
    • Notes
      1. Recommended Usage: Random Primer Mix (60 µM) contains 35 µM hexamers and 25 µM dT23VN  and 1 mM dNTPs. We recommend using Random Primer Mix at a final concentration of 6 µM.
    • References
      1. Goff, L.A. et al. (2004). BMC Genomics. 5, 76.
      2. Djikeng, A. et al. (2008). BMC Genomics. 9, 5.
      3. Rashtchian, A. (1994). Genome Research. 4, 83.
  • FAQs & Tech Tips
  • Protocols & Manuals
  • Quality & Safety
  • Legal Information
    • Legal And Disclaimers
      This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

      While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

      For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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