Random Primer Mix

Random priming is widely used in first strand cDNA synthesis.
Random Primer Mix is a ready-to-use optimized mixture of hexamers and anchored-dT primer (dT₂₃VN).
A mixture of hexamers and anchored-dT primer provides even and consistent coverage of the RNA template population across a wide range of RNA template concentration. In contrast, traditional random priming by hexamer has poor coverage of 3´ end of RNA templates (1,2,3).

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Catalog #	Concentration	Size	List Price	Your Price
S1330S	60 µM	100 µl	$97.00	Sign In Sign In

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Product Information
We recommend using Random Primer Mix for reverse transcription of the following RNA templates:
1. RNA without poly(A) tail such as ribosomal RNAs.
2. RNA with strong secondary structures.
3. Partially degraded RNA samples.
4. Target regions at 5´ end of a long messenger RNA transcript.
Figure 1:

Various amounts of human spleen total RNA (2 μg to 20 pg) were reverse transcribed using ProtoScript First Strand cDNA Synthesis Master Mix Kit (NEB #E6300 ) in the presence of 6 μM Random Primer Mix (panel A) or 3.5 μM random hexamers (panel B). About 1/10th of the cDNA products were then analyzed in duplicate qPCR reactions using a pair of primers specific for the beta actin gene with DyNAmo™ FLASH SYBR® Green qPCR Master Mix on a Bio-Rad IQ5® system.

Product Categories:

Reverse Transcriptases & RT-PCR Products

Applications:

RT-PCR & cDNA Synthesis,

RT-qPCR, RT-PCR and cDNA Synthesis
- Properties & Usage
  
  Storage Temperature
  -20°C
- Product Notes
  Recommended Usage: Random Primer Mix (60 µM) contains 35 µM hexamers and 25 µM dT₂₃VN and 1 mM dNTPs. We recommend using Random Primer Mix at a final concentration of 6 µM.
- References
  Goff, L.A. et al. (2004). BMC Genomics. 5, 76.
  
  Djikeng, A. et al. (2008). BMC Genomics. 9, 5.
  
  Rashtchian, A. (1994). Genome Research. 4, 83.
Protocols, Manuals & Usage
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  S1330Datasheet-Lot0041208
  
  S1330Datasheet-Lot0051211
  
  S1330Datasheet-Lot0061211
  
  S1330Datasheet-Lot0061312
  
  S1330Datasheet-Lot0071405
FAQs & Troubleshooting
- FAQs
  What products does NEB offer for functional genomics and reverse genetics?
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  S1330S_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  S1330S_v1_0081603
  
  S1330S_v1_0081606
  
  S1330S_v1_0091701
  
  S1330S_v1_0091709
  
  S1330S_v1_10021098
  
  S1330S_v1_10032777
  
  S1330S_v1_10045035
  
  S1330S_v1_10048074
  
  S1330S_v1_10050353
  
  S1330S_v1_10054265
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Random Primer Mix
- Datacards
  The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document.)
  
  S1330Datasheet-Lot0041208
  
  S1330Datasheet-Lot0051211
  
  S1330Datasheet-Lot0061211
  
  S1330Datasheet-Lot0061312
  
  S1330Datasheet-Lot0071405
- Legal and Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.