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Home RNA Reagents Products Random Primer Mix

Random Primer Mix

Random priming is widely used in first strand cDNA synthesis.
Random Primer Mix is a ready-to-use, optimized mixture of 35 µM hexamers, 25 µM dT₂₃VN, and 1 mM dNTPs.
A mixture of hexamers and anchored-dT primer provides even and consistent coverage of the RNA template population across a wide range of RNA template concentration. In contrast, traditional random priming by hexamer has poor coverage of 3´ end of RNA templates (1,2,3).
A final concentration of 6 µM is recommended.

Catalog #	Concentration	Size	List Price	Your Price
S1330S	60 µM	100 µl	$109.00	Sign In Sign In

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Product Information
We recommend using Random Primer Mix for reverse transcription of the following RNA templates:
1. RNA without poly(A) tail such as ribosomal RNAs.
2. RNA with strong secondary structures.
3. Partially degraded RNA samples.
4. Target regions at 5´ end of a long messenger RNA transcript.
Figure 1:

Various amounts of human spleen total RNA (2 μg to 20 pg) were reverse transcribed using ProtoScript First Strand cDNA Synthesis Master Mix Kit (NEB #E6300 ) in the presence of 6 μM Random Primer Mix (panel A) or 3.5 μM random hexamers (panel B). About 1/10th of the cDNA products were then analyzed in duplicate qPCR reactions using a pair of primers specific for the beta actin gene with DyNAmo™ FLASH SYBR® Green qPCR Master Mix on a Bio-Rad IQ5® system.

This product is related to the following categories:

Reverse Transcriptases & RT-PCR Products

This product can be used in the following applications:

RT-PCR & cDNA Synthesis,

RT-qPCR, RT-PCR and cDNA Synthesis
- Reagents Supplied
  Reagents Supplied
  
  The following reagents are supplied with this product:
  
  NEB # Component Name Component # Stored at (°C) Amount Concentration
  
  S1330S -20
  
  Random Primer Mix S1330SVIAL -20 1 x 0.1 ml 60 µM
- Properties & Usage
- Product Notes
  Recommended Usage: Random Primer Mix (60 µM) contains 35 µM hexamers and 25 µM dT₂₃VN and 1 mM dNTPs. We recommend using Random Primer Mix at a final concentration of 6 µM.
- References
  Goff, L.A. et al. (2004). BMC Genomics. 5, 76.
  
  Djikeng, A. et al. (2008). BMC Genomics. 9, 5.
  
  Rashtchian, A. (1994). Genome Research. 4, 83.
Citations & Technical Literature
- Citations
  
  Product Citation Tool
  
  Powered by Bioz See more details on Bioz
Quality, Safety & Legal
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications & Change Notifications
  Specifications & Change Notifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  S1330S_v1
  
  S1330S_v2
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  S1330S_v1_0081603
  
  S1330S_v1_0081606
  
  S1330S_v1_0091701
  
  S1330S_v1_0091709
  
  S1330S_v1_10021098
  
  S1330S_v1_10032777
  
  S1330S_v1_10045035
  
  S1330S_v1_10048074
  
  S1330S_v1_10050353
  
  S1330S_v1_10054265
  
  S1330S_v1_10061444
  
  S1330S_v2_10068574
  
  S1330S_v2_10071271
  
  S1330S_v2_10075360
  
  S1330S_v2_10080033
  
  S1330S_v2_10083161
  
  S1330S_v2_10085119
  
  S1330S_v2_10092411
  
  S1330S_v2_10096287
  
  S1330S_v2_10099965
  
  S1330S_v2_10101414
  
  S1330S_v2_10104362
  
  S1330S_v2_10106984
  
  S1330S_v2_10109708
  
  S1330S_v2_10116777
  
  S1330S_v2_10119051
  
  S1330S_v2_10121021
  
  S1330S_v2_10135527
  
  S1330S_v2_10138830
  
  S1330S_v2_10150178
  
  S1330S_v2_10163288
  
  S1330S_v2_10171314
  
  S1330S_v2_10182541
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Random Primer Mix
- Legal and Disclaimers
  
  Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
  
  New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.