Nickel Purification (His-tag)
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FAQs for Nickel Purification (His-tag)
- Which E. coli strain do you recommend to reduce contaminating proteins?
- What are to the advantages of performing purification under denaturing conditions?
- How much lysate can be loaded onto a single NEBExpress Ni Spin Column?
- Can NEBExpress® Ni Resin be used in large scale, pressurized purification systems such as FPLC?
Protocols for Nickel Purification (His-tag)
- NEBExpress® Ni Spin Columns Quick Start Protocol (NEB #S1427)
- NEBExpress® Ni Spin Column Reaction Protocol (NEB #S1427)
- NEBExpress® Ni Resin Quick Start Protocol (NEB #S1428)
- NEBExpress® Ni Resin Gravity Flow Typical Protocol (NEB #S1428)
- NEBExpress® Ni Resin Batch Binding Typical Protocol (NEB #S1428)
- NEBExpress® Ni Resin Pressurized Column Typical Protocol (NEB #S1428)
- Quick Start Protocol for NEBExpress® Ni-NTA Magnetic Beads
- NEBExpress® Ni-NTA Magnetic Beads Typical Reaction Protocol
- His-tag removal from protein using TEV Protease
- Protein Expression & Purification Brochure
- Purification Beads, Columns and Resins Brochure
- Purification Beads, Columns and Resins
Tools & Resources
Brochures
Selection Tools
NEBExpress Ni Spin Column Quick Start Protocol Schematic
Legal Information
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
