HomeFAQsWhich E. coli strain do you recommend to reduce contaminating proteins?
FAQ: Which E. coli strain do you recommend to reduce contaminating proteins?
Several E.coli proteins are capable of binding immobilized metal affinity matrices with moderate to high affinity. The most common contaminants include SlyD (28 KDa), GlmS (67 KDa), ArnA (74 KDa) and carbonic anhydrase (25 KDa). More information can be found in the following reference: Bolanos-Garcia et al. (2006) BBA 1760, 1304-1313. The named contaminants may be eliminated from the Ni-NTA elution fraction by employing NiCo21(DE3) Competent E. coli (NEB# C2529H) as the expression host and then performing a second binding step with chitin magnetic beads (NEB# E8036).
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
