Protein Expression
Choose Type:
- Quick Start Protocol for NEBExpress® Cell-free E. coli Protein Synthesis System
- Protein Synthesis Reaction using PURExpress (E6800)
- NEBExpress MBP Fusion and Purification System Quick Start Protocol (NEB #E8201)
- Simplified Expression and Purification Protocol (E6901)
- Protein Expression using the K. lactis Protein Expression Kit - Simultaneous Expression of Multiple Proteins
- Transformation of SHuffle® Competent Cell Strains
- E coli Lemo21 DE3 A T7 RNA Polymerase-based protein overexpression platform for routine and difficult targets
- Co-expression of Multiple Proteins in Kluyveromyces lactis
- Protein Expression with T7 Express Strains
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
- NEBExpress® Cell-free E. coli Protein Synthesis System
- Scaling down to scale up – Miniaturizing cell-free protein synthesis reactions with the Echo 525 Acoustic Liquid Handler
- Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
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Why Choose the K. lactis Protein Expression Kit?
Review the advantages of the K. lactis Protein Expression Kit for rapid, high yield protein expression in yeast.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Purification Beads, Columns and Resins Brochure
- Competent Cell Product Comparison
- IMPACT™ Vectors and Applications
- Protein Expression and Purification Selection Chart
- NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-tagged Recombinant Protein (2015)
- Development of a Rapid Method for Genome Engineering in a Quest Towards Customized Protein Expression Strains (2015)
- Discovering the Maltose Binding Protein epitope tag for Escherichia coli expressed proteins (2018)
- Selection for proteins that overcome heatinduced lethality of ΔdegP strain (2018)
- An E.coli Cell Lysate Based System for in vitro Protein Synthesis
- An E.coli lysate-based system for in vitro Protein Synthesis
Feature Articles
Brochures
Selection Tools
Posters
- Bhat, R. K. and Berger, S. (2007) New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii Prep Biochem Biotechnol; 37, 13-26. PubMedID: 17134979
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
