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  • Sodium Orthovanadate (Vanadate)

    Description

    Sodium Orthovanadate (Vanadate, Na3VO4) is a commonly used general inhibitor for protein phosphotyrosyl phosphatases (PTPs). It is a competitive inhibitor. The inhibition by Vanadate is completely reversible upon the addition of EDTA or by dilution. Vanadate is activated for maximal inhibition of PTPs following the procedure described by J.A.Gordon (1).

    The PTP activities are conveniently separable from the protein phosphoseryl and phosphothreonyl phosphatase (PSP) activities inhibited by Fluoride (NEB# P0759) and EDTA. Routinely Vanadate is used to preserve the protein tyrosyl phosphorylation state in cells, cell lysates, and protein tyrosine kinase assays (1, 2). 

    Properties and Usage

    Storage Temperature

    -20°C

    Notes

    1. Common buffer components such as EDTA and reducing agents may interact with Vanadate, affecting its potency (2).

      100% of inhibition of TC PTP (NEB #P0752) is found when NEBuffer for PTP (NEB #B0760S) is supplemented with 5 mM Vanadate.There is no inhibition of the same PTP in the presence of 50 mM Fluoride under the same condition (3).
    2. Storage: Sterile purified water adjusted to pH 10.0 (1,2).

    References

    1. Gordon, J.A. (1991). Methods in Enzymology. 201, 477-482.
    2. Huyer, G. et al. (1997). J. Biol. Chem.. 272, 843-851.
    3. Barshevsky, T. unpublished results .

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.