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DNA Gel Purification

The Monarch DNA Gel Extraction Kit reliably purifies up to 5 µg of concentrated, high quality DNA from agarose gels. It utilizes a bind/wash/elute workflow with minimal incubation and spin times. Our unique column design eliminates buffer retention and carryover of contaminants, enabling elution in volumes as low as 6 µl and with fewer steps. Monarch Buffers have been optimized, and do not require pH monitoring. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving a step. Eluted DNA is ready for use in restriction digestion, DNA sequencing, PCR, ligation, and other enzymatic manipulations.

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Monarch DNA Gel Extraction Kits provide fast and reliable purification, reduced hands-on time and elution in low volumes


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Our unique column design offers several improvements over other commercially available products, including the elimination of buffer retention and elution in as little as 6 µl

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FAQs for DNA Gel Purification

Protocols for DNA Gel Purification

Product Manual

Advantages of the Monarch DNA Gel Extraction Kit

  • Elute in as little as 6 μl
  • Prevent buffer retention and salt carryover with optimized column design
  • Save time with fast, user-friendly protocols
  • No need to monitor pH or add isopropanol
  • Buffers and columns available separately
  • Significantly less plastic used when compared with other kits
  • Responsibly-sourced and recyclable packaging

Specifications of the Monarch DNA Gel Extraction Kit

  • Binding Capacity: up to 5 μg
  • DNA Size Range: ~50 bp to 25 kb
  • Typical Recovery:
    DNA (50 bp to 10 kb): 70–90%
    DNA (11–25 kb): 50–70%
  • Elution Volume: ≥ 6 μl
  • Purity: A260/280 ≥ 1.8
  • Protocol Time: 10 min of spin and incubation time.
  • Compatible Downstream Applications: ligation, restriction digestion, labeling and other enzymatic manipulations, library construction and DNA sequencing

Legal Information

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.