monarch-buttefly

DNA Gel Extraction

Monarch nucleic acid purification kits provide fast and reliable purification of high quality DNA from bacterial cultures, agarose gels, and enzymatic reactions using best-in-class silica column technology.

 

Monarch Spin DNA Gel Extraction products can purify up to 5 µg of concentrated, high-quality double-stranded DNA from agarose gels. These products utilize a bind/wash/elute workflow with minimal incubation and spin times.

Our unique column design help eliminate buffer retention and carryover of contaminants, enabling elution in volumes as low as 5 µl and with fewer steps. Monarch Buffers have been optimized, and do not require pH monitoring. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving a step. Eluted DNA is ready for use in restriction digestion, DNA sequencing, PCR, ligation, and other enzymatic manipulations. These kits are effective for a wide range of DNA sizes and features a rapid 10-minute protocol. It recovers DNA better than the leading supplier and is more highly concentrated for greater downstream utility.

 

Workflow for DNA gel extraction for nucleic acid purification

 

Choose Monarch Spin Kits for DNA gel extraction for a wide range of DNA sizes

Monarch Spin DNA Gel Extraction Kit is effective for a wide range of DNA sizes

Gel photo showing broad size range

A mixture of 7 DNA fragments (lane I) ranging from 0.5 kb to 10 kb was prepared and resolved on a 1.2 % w/v TBE agarose gel. Each fragment was manually excised from the agarose gel and processed using the Monarch® Spin DNA Gel Extraction Kit. The elution of each fragment was resolved on a new gel with the original mixture for comparison.

 

 

 


Choose Product:

DNA Gel Extraction
Close

Monarch StabiLyse DNA/RNA Buffer

Close

Monarch® Buffer BY

Close

Monarch® Buffer WZ

Close

Monarch® Spin DNA Gel Extraction Kit

Close

β-Agarase I


Choose Type:

FAQs for DNA Gel Extraction
Protocols for DNA Gel Extraction
Legal Information

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

 


Videos

Learn about our
sustainability efforts

Green icon of recycling symbol
Explore our Monarch sustainability efforts.

View environmental sustainability

Learn about our
unique column design

Icon of nucleic acid purification column
Monarch columns offer improved performance.

 

View more about our columns

See how Monarch compares

Icon of bar graph and trend line representing performance data Monarch kits offer nucleic acid purifications solutions at the same or better quality than the leading supplier.

View data and testimonials