DNA Gel Extraction
Monarch Spin DNA Gel Extraction products can purify up to 5 µg of concentrated, high-quality double-stranded DNA from agarose gels. These products utilize a bind/wash/elute workflow with minimal incubation and spin times.
Our unique column design help eliminate buffer retention and carryover of contaminants, enabling elution in volumes as low as 5 µl and with fewer steps. Monarch Buffers have been optimized, and do not require pH monitoring. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving a step. Eluted DNA is ready for use in restriction digestion, DNA sequencing, PCR, ligation, and other enzymatic manipulations. These kits are effective for a wide range of DNA sizes and features a rapid 10-minute protocol. It recovers DNA better than the leading supplier and is more highly concentrated for greater downstream utility.
Choose Monarch Spin Kits for DNA gel extraction for a wide range of DNA sizes
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- What type of agarose gels are compatible with the Monarch® Spin DNA Gel Extraction Kit (NEB #T1120)?
- Can I purchase additional Monarch® columns and collection tubes for the MonarchSpin DNA Gel Extraction Kit (NEB #T1120) and the Monarch Spin PCR & DNA Cleanup Kit (NEB #T1130) separately?
- Can the Monarch PCR & DNA Cleanup Kit (5 μg) be used to purify RNA?
- What is the smallest volume of elution buffer that can be used with the Monarch® Spin Columns S1A (NEB #T2037)?
- What is the maximum binding capacity of the Monarch® Spin Columns S1A provided in the Monarch Spin DNA Gel Extraction Kit (NEB #T1120)?
- Can I excise a fragment from a gel and store it for purification with the Monarch® Spin DNA Gel Extraction Kit (NEB #T1120) at a later time?
- What size DNA can be purified with the Monarch® Spin Columns S1A (NEB #T2037)?
- Are Monarch® Spin Columns S1A (NEB #T2037) compatible with vacuum manifolds?
- Buffer Preparation Guidance for Monarch® Spin DNA Gel Extraction Kit (NEB #T1120)
- Standard Gel Extraction Protocol using the Monarch® Spin DNA Gel Extraction Kit and a Vacuum Manifold (NEB #T1120)
- Standard Gel Extraction Protocol using the Monarch® Spin DNA Gel Extraction Kit and Centrifugation (NEB #T1120)
- Monarch DNA Purification Brochure
- Troubleshooting Guide for DNA Cleanup and Plasmid Purification
- Back to basics: Important things to keep in mind when purifying plasmids and DNA fragments
- Six Tips for a Perfect Gel Extraction
Brochures
Troubleshooting Guides
Usage Guidelines
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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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Monarch kits offer nucleic acid purifications solutions at the same or better quality than the leading supplier.
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