pTXB1 Vector

pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.

  • View sequence details
  • Used in the IMPACT™ Protein Purification System
  • Recommended for use in intein-mediated protein ligation and C-terminal labeling
  • Double-stranded vector; 6,706 base pairs in length
  • Ampicillin resistance

Ordering Information

N6707S_pdp_800X450
  • 200 μg/ml
    10 μg
    $121.00
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    IMPACT Citations

    pTXB1 is an E. coli expression vector in the IMPACT™ Kit (NEB #E6901) (1,2). It is designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain (27 kDa) (2,3,4,5). The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein. pTXB vectors are recommended for use in intein-mediated protein ligation and C-terminal labeling (2). This double stranded vector is 6,706 base pairs in length.

    DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

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    E Coli Protein Expression
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