pTXB1 Vector

pTXB1 is an E. coli expression vector designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain. The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein.

  • Used in the IMPACT™ Protein Purification System
  • Recommended for use in intein-mediated protein ligation and C-terminal labeling
  • Double-stranded vector; 6,706 base pairs in length
  • Ampicillin resistance

Ordering Information

$name
  • 200 μg/ml
    10 μg
    $110.00
  • Product Information
    IMPACT Citations

    pTXB1 is an E. coli expression vector in the IMPACT™ Kit (NEB #E6901) (1,2). It is designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain (27 kDa) (2,3,4,5). The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein. pTXB vectors are recommended for use in intein-mediated protein ligation and C-terminal labeling (2). This double stranded vector is 6,706 base pairs in length.

    DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

    Product Categories:
    IMPACT™ Products,
    DNA Plasmid Products
    Applications:
    E.coli Protein Expression
    • Advantages and Features
    • Properties and Usage
    • Related Products
    • References
  • FAQs & Tech Tips
  • Protocols & Manuals
  • Other Tools & Resources
  • Quality & Safety
  • Legal Information
    • Legal And Disclaimers
Loading Spinner