M13mp18 RF I DNA

Description

M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the β-Galactosidase gene (1). When a fragment of DNA is inserted into one of these sites, the β-Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate (2).

DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.

Product Source

The phage M13mp18 is propagated in E. coli ER2738(3). The replicative form of DNA is isolated from infected cells and purified by a standard plasmid purification procedure. The final preparation is tested for its suitability as a vector.

Properties and Usage

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

References

  1. Norrander, J., Kempe, T. and Messing, J. (1983). Gene. 26, 101-106.
  2. Messing, J., Crea, R. and Seeburg, P.H. (1981). Nucl. Acids Res.. 9, 309-321.
  3. Messing, J. (1979). Recombinant DNA Technical Bulletin (NIH). 2, 43-48.

Protocols

  1. M13mp18 Phage Transfection

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.