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  • M13mp18 RF I DNA

    Description

    M13mp18 is the double-stranded, covalently closed, circular form of DNA derived from bacteriophage M13. This phage vector contains single HindIII, SphI, SbfI, PstI, SalI (AccI/ HincII), XbaI, BamHI, SmaI (XmaI), KpnI (Acc65I), SacI and EcoRI sites within the β-Galactosidase gene (1). When a fragment of DNA is inserted into one of these sites, the β-Galactosidase gene is inactivated, providing selection for clones on the appropriate indicator plate (2).

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Product Source

    The phage M13mp18 is propagated in E. coli ER2738(3). The replicative form of DNA is isolated from infected cells and purified by a standard plasmid purification procedure. The final preparation is tested for its suitability as a vector.

    Properties and Usage

    Affinity Tag

    Other

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Quality Control

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Functional Test (Transformation, Blue-White):

      Phage DNA was transformed into competent cells as undigested vector, linearized vector, linearized vector after ligation or linearized vector after ligation with target DNA.  Cells were plated onto plates containing X-gal and the number of blue and white plaques determined.

    References

    1. Norrander, J., Kempe, T. and Messing, J. (1983). Gene. 26, 101-106.
    2. Messing, J., Crea, R. and Seeburg, P.H. (1981). Nucl. Acids Res.. 9, 309-321.
    3. Messing, J. (1979). Recombinant DNA Technical Bulletin (NIH). 2, 43-48.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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