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  • Quick-Load® 1 kb Extend DNA Ladder

    Description

    The Quick-Load 1 kb Extend DNA ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing bromophenol blue dye as a tracking dye.

    The DNA Ladder consists of proprietary plasmids and DNA molecules which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 48.5 kilobases. The 3 kb fragment has increased intensity to serve as reference band.

    N3239

    Properties and Usage

    Bases

    FragmentMassbp
    13048,502
    2a1520,066
    2b1519,871
    34515,000
    43310,002
    5338,001
    6406,001
    7335,001
    8264,001
    91003,001
    10372,000
    11271,500
    12331,000
    13a18517
    13b17500

    Preparation

    The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

    Effective Size Range

    500bp to 48,502bp

    Storage Temperature

    4°C

    Storage Conditions

    2.5% Ficoll®-400
    11 mM EDTA
    3.3 mM Tris-HCl
    0.017% SDS
    0.015% bromophenol blue
    pH 8.0 @ 25°C

    Notes

    1. Quick-Load 1 kb Extend DNA Ladder is stable for at least 6 months at 25°C.

      Store at 4°C. Do not store at -20°C.

    2. Usage Recommendation: We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb Extend DNA Ladder per gel lane. For optimum separation of the highest molecular weight DNA fragments, run on a 0.6% (1X TAE or 1X TBE) agarose gel at 3.5 V/cm (distance between electrodes) for 3 hours.

      In general, it is recommended to load 1 ul per mm of gel lane.

      The Quick-Load 1 kb Extend DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.(Ed.), Molecular Cloning: A Laboratory Manual. pp. 10.51-10.67.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
    2. Can I use GelRed with the DNA Ladders from NEB?
    3. Can I use SYBR® with the DNA Ladders from NEB?