Quick-Load® 1 kb Extend DNA Ladder

Description

The Quick-Load 1 kb Extend DNA ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing bromophenol blue dye as a tracking dye.

The DNA Ladder consists of proprietary plasmids and DNA molecules which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 48.5 kilobases. The 3 kb fragment has increased intensity to serve as reference band.

NEB also offers a Quick-Load version of this ladder with purple dye.

N3239

Properties and Usage

Bases

FragmentMassbp
13048,502
2a1520,066
2b1519,871
34515,000
43310,002
5338,001
6406,001
7335,001
8264,001
91003,001
10372,000
11271,500
12331,000
13a18517
13b17500

Preparation

The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

Effective Size Range

500bp to 48,502bp

Storage Temperature

4°C

Storage Conditions

2.5% Ficoll®-400
11 mM EDTA
3.3 mM Tris-HCl
0.017% SDS
0.015% bromophenol blue
pH 8.0 @ 25°C

Notes

  1. Quick-Load 1 kb Extend DNA Ladder isstable for at least 6 months at 25°C.

    Store at 4°C. Do not store at -20°C.

  2. Usage Recommendation: We recommend loading10 μl (0.5 μg) of Quick-Load 1 kb Extend DNALadder per gel lane. For optimum separation of thehighest molecular weight DNA fragments, run on a0.6% (1X TAE or 1X TBE) agarose gel at 3.5 V/cm(distance between electrodes) for 3 hours.

    In general, it is recommended to load 1 ul per mm of gel lane.

    The Quick-Load 1 kb Extend DNA Ladder is notintended for precise quantification of DNA mass butcan be used for approximating the mass of DNA incomparably intense samples of similar size.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.(Ed.), Molecular Cloning: A Laboratory Manual. pp. 10.51-10.67.

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. Can I use GelRed with the DNA Ladders from NEB?
  3. Can I use SYBR® with the DNA Ladders from NEB?

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.