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  • Fast DNA Ladder

    Description

    The Fast DNA ladder is a pre-mixed, ready-to-load molecular weight marker containing xylene cyanol FF dye as a tracking dye. 

    The DNA Ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight standards for fast electrophoresis systems as well as standard electrophoresis. The digested DNA includes fragments ranging from 50 base pairs to 10 kilobases. The 1 kb fragment has increased intensity to serve as reference band.

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    Fast DNA Ladder. Mass values are for 0.5 μg/lane

    Properties and Usage

    Bases

    FragmentMassbp
    13610,002
    2365,001
    3363,001
    4382,000
    5281,500
    61081,000
    743766
    840500
    933300
    1041150
    116150

    Effective Size Range

    50bp to 10,002bp

    Storage Temperature

    4°C

    Storage Conditions

    0.002% Xylene Cyanol
    5 mM Tris-HCl
    5 mM EDTA
    5% Glycerol
    pH 8.0 @ 25°C

    Notes

    1. Preparation: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
    2. Usage Recommendation: For standard electrophoresis applications, we recommend loading 20 µl (0.5 µg) of the Fast DNA Ladder per gel lane. The best separation occurs on a 1.2% agarose gel. Below 1%, the 50 bp fragment may not separate from the 150 bp fragment. For use on a fast electrophoresis system, follow the loading recommendations of the system’s manufacturer (5 to 20 µl). The Fast DNA Ladder is suitable for use on the FlashGel® System from Lonza (1.2% gel) or on the E-Gel® system from Invitrogen (1.2 or 2% gels). The Fast DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    3. Fast DNA Ladder is stable for at least 6 months at 25°C.
    4. For long term storage. Store at 4°C or -20°C. If stored at -20°C, mix well after thawing.

    References

    1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor(Ed.), Molecular Cloning: A Laboratory Manual, (2nd ed.). 10.51-10.67. Cold Spring Harbor Laboratory Press.

    FAQs

    1. How much Fast DNA Ladder should I load on a gel?
    2. What percentage of agarose gel should I use?
    3. Can I store it at -20°C?
    4. Can I use GelRed with the DNA Ladders from NEB?
    5. Can I use SYBR® with the DNA Ladders from NEB?
    6. Why are there extra bands visible on polyacrylamide gels?

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Selection Tools

    Certificate of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.