pBR322 DNA-MspI Digest

Description

The MspI digest of pBR322 DNA yields 26 fragments. Suitable for use as molecular weight standards for agarose gel electrophoresis (1,2).

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



N3032_thumb

pBR322 DNA-MspI Digest visualized by ethidium bromide staining on a 3% agarose gel.

Suggested Load: 1 µg/ gel lane. 

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Gel Loading Dye, Purple (6X), no SDS256X

Properties and Usage

Bases

FragmentMassbp
1143622
2121527
393404
470307
555242
655238
750217
846201
944190
1041180
11, 1274160
13, 1468147
1528123
1625110
172190
181776
191567
20, 211634
22, 231226
24315
25, 2649

Effective Size Range

9bp to 622bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Notes

  1. Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.

    1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Sutcliffe, J.G. (1978). Cold Spring Harbor Symp. Quant. Bio.. 43, 77-90.
  2. Peden, K.W.C. (1983). Gene. 22, 277-280.

FAQs

  1. Why is the separation of the lower bands incomplete?
  2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  3. How can I quantify the amount of DNA in each band of a marker?
  4. Can I use GelRed with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  6. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Protocols

  1. Suggested protocol for loading a DNA Ladder/marker

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Citations

  • Yang GQ, Chen YM, Wang JP, Guo C, Zhao L, Wang XY, Guo Y, Li L, Li DZ, Guo ZH. (2016). Development of a universal and simplified ddRAD library preparation approach for SNP discovery and genotyping in angiosperm plants. Plant Methods. . Aug 4;12, 39.

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.