pBR322 DNA-BstNI Digest

Description

The BstNI Digest of pBR322 DNA yields 6 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1,2).

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



N3031_thumb

pBR322 DNA-BstNI Digest visualized by ethidium bromide staining. 1.4% agarose gel.
The full list of DNA fragments can be found in the chart below.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Gel Loading Dye, Purple (6X), no SDS256X

Properties and Usage

Bases

FragmentMassbp
14261,857
22431,058
3213929
488383
528121
6313

Effective Size Range

13bp to 1,857bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Notes

  1. For long term storage, store at -20°C. If samples need to be diluted, use TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.

    1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Sutcliffe, J.G. (1978). Cold Spring Harbor Symp. Quant. Bio.. 43, 77-90.
  2. Peden, K.W.C. (1983). Gene. 22, 277-280.

FAQs

  1. Why is the separation of the lower bands incomplete?
  2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  3. How can I quantify the amount of DNA in each band of a marker?
  4. Can I use GelRed with the DNA Ladders from NEB?
  5. Can I use Midori Green with the DNA Ladders from NEB?
  6. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Protocols

  1. Suggested protocol for loading a DNA Ladder/marker

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.