ΦX174 RF II DNA
- Commonly used cloning vector
- Double-stranded nicked circular form of PhiX174 - relaxed form
- Molecular Weight is 3.5 x106 Daltons
- 5,386 base pairs in length
- Greater then 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ⌽X174 DNA
Product SourceΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II form as indicated by agarose gel electrophoresis.
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