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  • ΦX174 RF II DNA


    This is the double-stranded, nicked, circular form of ΦX174 DNA (relaxed form). The molecular weight of ΦX174 DNA is 3.50 X 106 daltons it is 5,386 base pairs in length. Greater than 90% of the molecules are RF II, the remainder are RF I (supercoiled) and linear ΦX174 DNA.

    DNASU is a central repository for plasmid clones and collections that may also be helpful.

    Product Source

    ΦX174 RF II is prepared by relaxing (nicking) ΦX174 RF I DNA in high salt at 75°C. Greater than 90% of the molecules are in the RF II form as indicated by agarose gel electrophoresis.

    Properties and Usage

    Affinity Tag


    Storage Temperature


    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C


    1. Sanger, F. et al. (1978). J. Mol. Biol.. 125, 225-246.
    2. Freifelder, D. and Dewitt, R. (1977). Gene. 1, 385.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.


    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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