λ DNA-Mono Cut Mix

Description

The Mono Cut Mix is a mixture of intact lambda DNA and lambda DNA separately digested with ApaI, KpnI, XbaI and XhoI. The fragments have been filled in with DNA Polymerase I Large (Klenow) Fragment to prevent re-annealing. These fragments are best separated by Pulsed Field Gel Electrophoresis.The approximate mass of DNA in each of the bands is provided (assuming a 0.5 μg load) for approximating the mass of DNA in comparably intense samples of similar size.

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.



N3019_thumb

PFGE separation of 0.5 µg of Lambda Mono Cut Mix. 1% agarose gel, 0.5X TBE, 6 V/cm, 15°C for 20 hours. Switch times ramped from 0.5-1.5 seconds.

Reagents Supplied

The following reagents are supplied with this product:

Store at (°C)Concentration
Gel Loading Dye, Purple (6X), no SDS256X

Properties and Usage

Bases

FragmentMassbp
110048502
27938416
36933498
46229946
55124508
64923994
73517053
83115004
92110086
1031503

Effective Size Range

1,503bp to 48,502bp

Storage Temperature

-20°C

Storage Conditions

10 mM Tris-HCl
1 mM EDTA
pH 8.0 @ 25°C

Notes

  1. These fragments are best separated by Pulsed Field Gel Electrophoresis. Alternatively Lambda Mono Cut Mix can be separated by conventional gel electrophoresis using the following conditions: 0.5 μg Lambda Mono Cut Mix, 0.4 % agarose gel, 1X TAE, 20-30 V, 25°C for 24 hours.
  2. 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Daniels, D.L. et al. (1983). Appendix II: Complete Annotated Lambda Sequence. R.W. Hendrix, J.W. Roberts, F.W. Stahl and R.A. Weisberg(Ed.), Lambda-II. 519-676. New York: Cold Spring Harbor Laboratory Press.

FAQs

  1. How can I quantify the amount of DNA in each band of a marker?
  2. General information on Lambda DNA Mono Cut Mix:
  3. Can I use GelRed with the DNA Ladders from NEB?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® with the DNA Ladders from NEB?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Protocols

  1. Suggested protocol for loading a DNA Ladder/marker

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Certificate of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality control's for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.