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  • Supercoiled DNA Ladder

    Description

    The Supercoiled DNA ladder contains 9 proprietary supercoiled plasmids, ranging in size from 2 to 10 kb, that are suitable for use as supercoiled molecular weight standards for agarose electrophoresis. The 5 kb plasmid has an increased intensity to serve as a reference band.

    Comes supplied with 1 vial of Gel Loading Dye, Blue (6X).



    N0472_thumb

    0.5 µg of Supercoiled DNA Ladder visualized by ethidium bromide staining on a 0.8% TAE agarose gel.

    Properties and Usage

    Bases

    FragmentMassbp
    14510000
    2458000
    3456000
    41365000
    5454000
    6453500
    7453000
    8452500
    9452017

    Effective Size Range

    2,017bp to 10,000bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. This ladder may contain some traces of nicked DNA and dimers above the 10 kb plasmid. To minimize nicking of the supercoiled DNA, always use sterile pipette tips and avoid multiple freeze-thaw cycles. The migration of supercoiled plasmids in agarose gels can change depending on agarose concentration, buffer and electrophoresis conditions. Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH20
    2. The 9 proprietary plasmids are purified, phenol extracted and equilibrated to 10 mM Tris-HCl (pH 8.0) and 1 mM EDTA.
    3. Centrifuge briefly and mix gently before use. We recommend loading 0.5 µg (1 µl) of the Supercoiled DNA Ladder diluted in sample buffer. This ladder was not designed for precise quantification of DNA mass, but can be used for approximating the mass of DNA in comparably intense samples of similar size.
    4. Storage: Dividing in several aliquots is recommended to avoid multiple freeze-thaw cycles.

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press. (2nd ed.)

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Can I use GelRed with the DNA Ladders from NEB?
    2. Can I use SYBR® with the DNA Ladders from NEB?
    3. Can I use Midori Green with the DNA Ladders from NEB?
    1. Suggested Loading a Sample (N0472)

    We recommend aliquoting, to avoid too many freeze-thaw cycles.