Low Range ssRNA Ladder

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N0364S     -20       Low Range ssRNA Ladder N0364SVIAL -20 1 x 0.05 ml Not Applicable   RNA Loading Dye, (2X) B0363AVIAL -20 1 x 1 ml 2 X

Companion Products

• microRNA Marker
• RNA Loading Dye, (2X)

Materials Sold Separately

• RNA Loading Dye, (2X)

1. Minimize repeated freeze-thawcycles. It is best to aliquot the marker into singleuse portions.
   
   To avoid ribonuclease contamination: wear gloves,use RNase-free water for gels and buffers, washequipment with detergent and rinse thoroughlywith RNase-free water.
   
   
2. Store at -20˚C for long-term storage. For short-term storage (<1 week), store at 4˚C.

1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. 2nd ed., 743-745.
2. Liu, Y-C. and Chou, Y-C. (1990). Biotechniques. 9,
3. Sandra Cook, and Christina Marchetti Unpublished observation
4. Dong Ma, and John Greci Unpublished observation

1. Sample preparation using provided buffer (N0364)

1. I need to know what types of gel to use to run RNA ladders?
2. What types of gel and staining dyes can I use for NEB’s Low Range ssRNA Ladder (N0364S)?
3. Why the bands of the Low Range ssRNA Ladder on my 2% agarose-TBE gel are not resolved well?
4. Why do I have extra bands of the Low Range ssRNA Ladder on my denaturing 6% TBE-Urea gel?
5. Will the Low Range ssRNA Ladder run on 10% TBE-Urea and 15% TBE-Urea gels?
6. What is the concentration of RNA in the ssRNA Ladder (NEB #N0362) and Low Range ssRNA Ladder (NEB #N0364)?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications & Change Notifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• N0364S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N0364S_v1_0271601
• N0364S_v1_0271605
• N0364S_v1_0281609
• N0364S_v1_0281704
• N0364S_v1_0281709
• N0364S_v1_0281802
• N0364S_v1_10013508
• N0364S_v1_10018990
• N0364S_v1_10024418
• N0364S_v1_10031678
• N0364S_v1_10041516
• N0364S_v2_10052279
• N0364S_v2_10056647
• N0364S_v2_10062652
• N0364S_v2_10069539
• N0364S_v2_10077426
• N0364S_v2_10080102
• N0364S_v2_10082477
• N0364S_v2_10095257
• N0364S_v2_10105450
• N0364S_v2_10116075
• N0364S_v2_10134342
• N0364S_v2_10140334
• N0364S_v2_10149110
• N0364S_v2_10162765
• N0364S_v2_10175912

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Low Range ssRNA Ladder
• RNA Loading Dye, (2X)

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.