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  • dsRNA Ladder

    Description

    The dsRNA Ladder is a set of 7 annealed double-stranded RNA molecules produced by in vitro transcription of 14 linear DNA templates. The ladder sizes are: 500, 300, 150, 80, 50, 30 and 21 base pairs. All have 2-base, 3´ extensions. The 80 base pair fragment is at double intensity to serve as a reference point.

    This ladder is suitable for use as a size standard in dsRNA and RNAi analysis on both non-denaturing polyacrylamide and agarose gels.

    N0363_v1_000009

    Left: 1 µg of dsRNA Ladder was visualized by ethidium bromide staining on a 6% Polyacrylamide gel 10 V/cm.
    Right: 1.5 µg of dsRNA Ladder was visualized by ethidium bromide staining on a 2% TBE agarose gel 10 V/cm.

    Properties and Usage

    Bases

    Fragmentbp
    1500
    2300
    3150
    480
    550
    630
    721

    Effective Size Range

    21bp to 500bp

    Storage Temperature

    -20°C

    Storage Conditions

    10 mM Tris-HCl
    1 mM EDTA
    pH 8.0 @ 25°C

    Notes

    1. This marker was not designed for precise quantification of dsRNA mass.
    2. Double-stranded RNA is sensitive to degradation by ribonucleases, even though it is more resistant than single-stranded RNA.

      To avoid ribonuclease contamination: wear gloves, use RNase-free water for gels and buffers, wash equipment with detergent and rinse throughly with RNase-free water.

      Excessively long run times or high voltage can cause degradation of the bands on the gel. We recommend loading 1.0-1.5 µg of dsRNA Ladder, a running voltage of 4-10 V/cm , adding ethidium bromide to gels and running buffer at a final concentration of 0.5 µg/ml to effectively stain the bands during agarose gel electrophoresis.

    References

    1. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), (p. 7.43?7.45)
    2. Liu, Y-C. and Chou, Y-C. (1990). Biotechniques . 9
    3. Dong Ma unpublished observation.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.
    1. Can I use SYBR® with the DNA Ladders from NEB?
    dsRNA is difficult to completely denature. For sizing ssRNA in this range use the Low Range ssRNA Ladder N0364S and/or the microRNA marker N2102.