MidRange PFG Marker

Reagents Supplied

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
N0342S     -20       MidRange PFG Marker (GelSyringe Dispenser) N0342SVIAL -20 1 x 50 gel lanes 50 µg/ml

Companion Products

• λ DNA-Mono Cut Mix
• Lambda PFG Ladder

1. The photograph represents the pulsed field gel separation of Mid Range PFG Marker using a CHEF apparatus. The 1% agarose gel was run at 6 volts/cm using ramped pulse times from 1 to 25 seconds for 24 hours at 15°C in 0.5X TBE (50 mM Tris-HCl, 50 mM boric acid, 1 mM EDTA) made with Milli-Q™ water.
2. Extrude agarose from GelSyringe carefully and slice plugs from the end with a sharp blade. One plug is sufficient for one lane of a gel. Place the plug at the front of the well and seal with molten agarose just above gelling temperature (~42–45°C). Allow no bubbles to form.
3. Recommended plug sizes are from 5-10 µl. A 10 µl plug (one small graduation on the GelSyringe volume scale) contains approximately 0.5µg of DNA. Each GelSyringe yields 50+ plugs.
4. Melting plugs will cause denaturation of concatemers.
5. Never attach the agarose plugs to the gel comb before the gel is poured. Heat from the solidifying gel will cause the Lambda concatemers to denature.

1. Ellard, J., Greci, J. and Davis, T.B. unpublished observation.

1. Why am I seeing poor results when running the PFG marker on my pulse field gel?

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• N0342S_v1_10021921
• N0342S_v1_10027632
• N0342S_v1_10050252
• N0342S_v1_10070520
• N0342S_v1_10089555
• N0342S_v1_10123745
• N0342S_v1_10146952

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• MidRange PFG Marker

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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