USER® Enzyme

A thermolabile version is also available, Thermolabile USER II Enzyme (NEB #M5508).

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USER (Uracil-Specific Excision Reagent) Enzyme (1) generates a single nucleotide gap at the location of a uracil residue.

Applications of USER include:

  • Directional RNA Seq
  • NEBNext adaptor cleavage
  • USER cloning

 

USER Enzyme

Uracil DNA glycosylase (UDG) catalyzes the excision of a uracil base, forming an abasic (apyrimidinic) site while leaving the phosphodiester backbone intact. The lyase activity of Endonuclease VIII breaks the phosphodiester backbone at the 3´ and 5´ sides of the abasic site so that base-free deoxyribose is released. USER (Uracil-Specific Excision Reagent) Enzyme, combines these two enzymatic activities to generate a single nucleotide gap at the location of a uracil residue.


Ordering Information

M5505S_pdp_800X450
  • 1,000 units/ml
    50 units
    $72.00
  • 1,000 units/ml
    250 units
    $288.00
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