Nucleoside Digestion Mix
The Nucleoside Digestion Mix is an optimized mixture of enzymes that provides a convenient one-step method to generate single nucleosides from DNA or RNA for quantitative analysis by liquid chromatography-mass spectrometry (LC-MS), eliminating the need for sequential multi-step, time-consuming digestion protocols.
- Convenient one-step protocol
- Digests both DNA and RNA to single nucleosides
- Low-glycerol formulation significantly reduces glycerol-induced ion suppression during MS analysis
"I am very happy with the NEB Nucleoside Digestion Mix. Not only does it provide greater levels of digestion, but it allows me to degrade both DNA and RNA simultaneously."
– R.K., Assistant Professor, University of Pennsylvania
The Nucleoside Digestion Mix is a mixture of enzymes that provides a convenient one-step method to generate single nucleosides from DNA or RNA. Optimized for quantitative analysis by liquid chromatography-mass spectrometry (LC-MS), this reagent eliminates the need for sequential multi-step, time-consuming digestion protocols. The Nucleoside Digestion Mix digests ssDNA, dsDNA, DNA/RNA hybrids and RNA (except mRNA cap structures) containing epigenetically modified (m5C, hm5C, f5C, ca5C, m4C, m6A, etc.), unnatural, or damaged bases. Moreover, the low-glycerol formulation (<1%) significantly reduces glycerol-induced ion suppression during mass spectrometry analysis.
Supplied in: 20 mM Tris-HCl (pH 7.5), 1 mM MgCl2, 2 mM CaCl2, 2 mM ZnCl2, 50 mM NaCl and 0.6% glycerol.
The following reagents are supplied with this product:
Store at (°C) Concentration Nucleoside Digestion Mix Reaction Buffer 10 X
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- Epigenetic Analysis (EpiMark® Validated) Products,
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- DNA Modifying Enzymes Products ,
- Nuclease Products
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