- 5' → 3' exonuclease
- Removal of 5' mononucleotides from DNA
T7 Exonuclease acts in the 5' to 3' direction, catalyzing the removal of 5' mononucleotides from duplex DNA. T7 Exonuclease is able to initiate nucleotide removal from the 5' termini or at gaps and nicks of double-stranded DNA (1). It will degrade both 5' phosphorylated or 5' dephosphorylated DNA. It has also been reported to degrade RNA and DNA from RNA/DNA hybrids in the 5' to 3' direction but is unable to degrade either double-stranded or single-stranded RNA (2). The protein is the product of T7 gene 6.
- Isolated from a recombinant source
- 5' -> 3' exonuclease
- Supplied with 10X Reaction Buffer
Product SourcePurified from an E. coli strain containing a TYB12 intein fusion.
The following reagents are supplied with this product:
Store at (°C) Concentration NEBuffer™ 4 -20 10 X
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