Lambda Exonuclease
- Highly processive 5' → 3' exonuclease
- Removal of 5' mononucleotides from duplex DNA
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Product Information
A highly processive enzyme that acts in the 5´ to 3´ direction, catalyzing the removal of 5´ mononucleotides from duplex DNA. The preferred substrate is 5´-phosphorylated double stranded DNA, although it will also degrade single-stranded and non-phosphorylated substrates at a greatly reduced rate. Lambda Exonuclease is unable to initiate DNA digestion at nicks or gaps (1). Highlights
Isolated from a recombinant source
Highly processive 5'→3' exonuclease
Supplied in 10X Reaction BufferProduct Source
A genetic fusion of the E. coli Lambda Exonuclease gene with the gene encoding maltose binding protein (MBP). Following affinity chromatography, Lambda Exonuclease is cleaved from the fusion construct and purified away from MBP.Reagents Supplied
The following reagents are supplied with this product:
Store at (°C) Concentration Lambda Exonuclease Reaction Buffer -20 10 X - Product Categories:
- Nuclease Products
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