Lambda Exonuclease

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. The activity of Lambda Exonuclease in various buffers is listed below:

   • NEBuffer 1 - 15%
   • NEBuffer 2 - 40%
   • NEBuffer 3 -10%
   • NEBuffer 4 - 40%
   • CutSmart – 50%
   • Exonuclease I Reaction Buffer- 100%
   • Thermopol Reaction Buffer -100%
2. 5’-OH ends are digested 20X slower than 5’-PO₄ ends. ssDNA is digested 100X slower than dsDNA.   

1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

1. Protocol for Lambda Exonuclease (M0262)

• Activity of DNA Modifying Enzymes in CutSmart® Buffer

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

• Choosing the Right Exonuclease (2019)

1. What is the activity of M0262 Lambda Exonuclease in various NEB buffers?
2. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
3. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
4. Will Lambda Exonuclease work in CutSmart Buffer?
5. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

Product Citation Tool

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0262S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0262L_v1_10013107
• M0262S_v1_10013141
• M0262L_v1_10026204
• M0262L_v1_10036383
• M0262S_v1_10042368
• M0262L_v1_10050139
• M0262S_v1_10050141
• M0262L_v1_10055887
• M0262S_v1_10055886
• M0262S_v1_10058614
• M0262L_v1_10059640
• M0262S_v1_10062809

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Lambda Exonuclease

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.