Unit Definition

One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [³H]-DNA.

Reaction Conditions

1X Lambda Exonuclease Reaction Buffer
Incubate at 37°C

1X Lambda Exonuclease Reaction Buffer
67 mM Glycine-KOH
2.5 mM MgCl₂
50 μg/ml BSA
(pH 9.4 @ 25°C)

Usage Concentration

5,000 - units/ml

Storage Temperature

-20°C

Storage Conditions

25 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Heat Inactivation

75°C for 10 min

Molecular Weight

Theoretical: 28000 daltons

Specific Activity

50,000 units/mg

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. The activity of Lambda Exonuclease in various buffers is listed below:

   • NEBuffer 1 - 15%
   • NEBuffer 2 - 40%
   • NEBuffer 3 -10%
   • NEBuffer 4 - 40%
   • CutSmart – 50%
   • Exonuclease I Reaction Buffer- 100%
   • Thermopol Reaction Buffer -100%

1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

1. What is the activity of M0262 Lambda Exonuclease in various NEB buffers?
2. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?
3. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
4. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
5. Will Lambda Exonuclease work in CutSmart Buffer?

• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Endonucleases

• NEBioCalculator

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Lambda Exonuclease
• Lambda Exonuclease Reaction Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.