Unit Definition

One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [³H]-DNA.

Reaction Conditions

1X Lambda Exonuclease Reaction Buffer
Incubate at 37°C

1X Lambda Exonuclease Reaction Buffer
67 mM Glycine-KOH
2.5 mM MgCl₂
50 μg/ml BSA
(pH 9.4 @ 25°C)

Usage Concentration

5,000 - units/ml

Storage Temperature

-20°C

Storage Conditions

25 mM Tris-HCl
50 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8.0 @ 25°C

Heat Inactivation

75°C for 10 min

Molecular Weight

Theoretical: 28000 daltons

Specific Activity

50,000 units/mg