Lambda Exonuclease

The following reagents are supplied with this product:

NEB #	Component Name	Component #	Stored at (°C)	Amount	Concentration
M0262S     -20       Lambda Exonuclease M0262SVIAL -20 1 x 0.2 ml 5,000 units/ml   Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X
M0262L     -20       Lambda Exonuclease M0262LVIAL -20 1 x 1 ml 5,000 units/ml   Lambda Exonuclease Reaction Buffer B0262SVIAL -20 1 x 1.5 ml 10 X

1. The activity of Lambda Exonuclease in various buffers is listed below:

   • NEBuffer 1 - 15%
   • NEBuffer 2 - 40%
   • NEBuffer 3 -10%
   • NEBuffer 4 - 40%
   • CutSmart – 50%
   • Exonuclease I Reaction Buffer- 100%
   • Thermopol Reaction Buffer -100%
2. 5´-OH ends are digested 20X slower than 5´-PO₄ ends. ssDNA is digested 100X slower than dsDNA.   
3. Phosphorothioate (PT) bond is resistant to Lambda Exonuclease cleavage. However, only one of the two chiral forms of the PT bond is resistant. To achieve complete protection against degradation at all PT bond positions, it is recommended to include five consecutive PT bonds in chemically synthesized DNA. Learn more here.

1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

1. Protocol for Lambda Exonuclease (M0262)

• Activity of DNA Modifying Enzymes in rCutSmart™ Buffer

• Activities of Exonucleases and Non-specific Endonucleases
• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Non-specific Endonucleases

• Exo Selector

• Choosing the Right Exonuclease (2019)

1. What is the activity of M0262 Lambda Exonuclease in various NEB buffers?
2. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
3. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
4. Will Lambda Exonuclease work in rCutSmart Buffer?
5. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

• The effect of nucleic acid modifications on digestion by DNA exonucleases

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• M0262S_L_v1

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• M0262L_v1_10013107
• M0262S_v1_10058614
• M0262S_v1_10069223
• M0262L_v1_10073288
• M0262L_v1_10094822
• M0262L_v1_10116024
• M0262S_v1_10182337
• M0262L_v1_10218709
• M0262L_v1_10246057
• M0262L_v1_10265399
• M0262S_v1_10282744

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.