Unit Definition

One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [³H]-DNA.

Reaction Conditions

IX Lambda Exonuclease Reaction Buffer
Incubate at 37°C

1X Lambda Exonuclease Reaction Buffer
50 μg/ml BSA
67 mM Glycine-KOH
2.5 mM MgCl₂
(pH 9.4 @ 25°C)

Usage Concentration

5,000 - units/ml

Storage Temperature

-20°C

Storage Conditions

0.1 mM EDTA
50% Glycerol
50 mM NaCl
25 mM Tris-HCl
1 mM DTT
pH 8.0 @ 25°C

Heat Inactivation

75°C for 10 min

Molecular Weight

Theoretical: 28000 daltons

Specific Activity

50,000 units/mg

Companion Products

• Monarch® Plasmid Miniprep Kit 
• Monarch® DNA Gel Extraction Kit
• Monarch® PCR & DNA Cleanup Kit (5 μg)

1. Little, J.W. (1981). Gene Amplif. Anal. 2, 135-145.

1. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?
2. How Does T5 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
3. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
4. Will Lambda Exonuclease work in CutSmart Buffer?

• Common Applications for Exonucleases and Endonucleases
• Properties of Exonucleases and Endonucleases

• NEBioCalculator

Quality Assurance Statement

• Purified free of contaminating endonucleases and exonucleases.

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• Lambda Exonuclease
• Lambda Exonuclease Reaction Buffer

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.