T4 RNA Ligase 2, truncated
icon_cloned icon_recomb icon_NEBU 25 degree incubation temp icon_HI_65

  • T4 RNA Ligase 2, truncated (T4 Rnl2tr) specifically ligates the pre-adenylated 5´ end of DNA or RNA to the 3´ end of RNA.
  • The enzyme does not require ATP for ligation but does need the pre-adenylated substrate.
  • T4 Rnl2tr is expressed from a plasmid in E. coli, which encodes the first 249 amino acids of the full length T4 RNA Ligase 2.
  • Unlike the full length ligase, T4 Rnl2tr cannot ligate the phosphorylated 5´ end of RNA or DNA to the 3´ end of RNA.
  • This enzyme, also known as Rnl2 (1–249) has been used for optimized linker ligation for the cloning of microRNAs.
  • This enzyme reduces background ligation because it can only use pre-adenylated linkers.
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Catalog # Concentration Size List Price Your Price Quantity
M0242S 200,000 units/ml 2,000 units $69.00
M0242L 200,000 units/ml 10,000 units $277.00
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