IsoAmp® II Universal tHDA Kit


Thermophilic Helicase-Dependent Amplification (tHDA) is a novel method for isothermal amplification of nucleic acids. Like PCR, the tHDA reaction selectively amplifies a target sequence defined by two primers. However, unlike PCR, tHDA uses an enzyme called a helicase to separate DNA, rather than heat. This allows DNA amplification without the need for thermocycling. The tHDA reaction can also be coupled with reverse transcription for RNA analysis.

IsoAmp® II Universal tHDA kit can be used to amplify and detect short DNA sequences (70 bp - 120 bp) at a constant temperature. The kit can be used with a variety of templates, including microbial genomic DNA, viral DNA, plasmid DNA, and cDNA. A single copy of target DNA can be amplified by tHDA and detected by agarose gel electrophoresis when optimized primers and buffer are used.

 IsoAmp® II Universal tHDA kit is based on a second-generation thermophilic Helicase-Dependent Amplification platform. The reactions supported by IsoAmp® II Universal tHDA kit include tHDA, reverse transcription HDA (RT-HDA), real-time quantitative HDA (qHDA) and real-time quantitative RT-HDA (qRT-HDA), from a single reaction buffer. IsoAmp® II Universal tHDA kit contains an enzyme mix, dNTP solution and buffer, allowing flexibility in reaction setup. A Control Template and a set of specific primers are supplied for the positive control reaction.

Recommended storage conditions for the tHDA kit are -20°C for storage shorter than 6 months and -80°C for storage greater than 6 months. Please avoid repeated freeze-thaw cycles as this may decrease performance of the kit.
Figure 1.

Figure 1.

HDA Technology.
Figure 2.
Figure 2
Positive control assays with varying amount of Control Template. A, two-step tHDA positive control with 10 pg - 1 ng Control Template. B, two-step qHDA positive control using 500 fg - 5 ng, in triplicate. 

Properties and Usage

Storage Temperature



  1. How to design the primer and select the amplicon for IsoAmp II Universal tHDA Kit?


  1. One-Step tHDA (thermostable HDA)
  2. One-Step RT-HDA (Reverse Transcription tHDA)
  3. One-Step qHDA (Real-time quantitative tHDA)
  4. One-Step qRT-HDA (Real-time quantitative RT-HDA)
  5. Two-Step tHDA (thermostable HDA)
  6. Two-Step RT-HDA (Reverse Transcription tHDA)
  7. Two-Step qHDA (Real-time quantitative tHDA)
  8. Two-Step qRT-HDA (Real-time quantitative RT-HDA)

Interactive Tools

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.