Ph.D.™-C7C Phage Display Peptide Library Kit
The Ph.D.-C7C Phage Display Peptide Library is based on a combinatorial library of random peptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.
Please click the link for Applications of the Ph.D. Phage Display Peptide Libraries.
Complexity: 1.2 x 109 transformants.
Panning with a pentavalent peptide library displayed on plll.
The following reagents are supplied with this product:
Store at (°C) Concentration E. coli K12 ER2738 -20 Streptavidin, lyophilized -20 Biotin -20 10 mM Ph.D.™-C7C Phage Display Peptide Library -20 1 x10<sup>13</sup> pfu/ml Streptavidin, lyophilized -20 Biotin 10 mM -28 glll Sequencing Primer (22-mer) -20 1 pmol/μl -96 glll Sequencing Primer (20-mer) -20 1 pmol/μl
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