Home Protein Tools Products Ph.D.™-C7C Phage Display Peptide Library Kit

Ph.D.™-C7C Phage Display Peptide Library Kit

The Ph.D.™-C7C Phage Display Peptide Library Kit contains a tube of the Ph.D.-C7C Phage Library, protein reagents for a control reaction and sequencing primer stocks. The Ph.D.-C7C library is a combinatorial library of random peptides with a disulfide constrained loop, fused to a minor coat protein (pIII) of M13 phage. The displayed peptide, in the form AC-X7-CGGGS, is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.

Ready to use complex phage peptide library
Structurally constrained peptide library
Applications and methodologies are numerous and supported by over 20 years of literature citations
Does not require helper phage for amplification
Inherent link between phenotype and genotype allows screening of billions of clones in a single microtiter well or Eppendorf tube

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Size

10 panning experiments

List Price

$610.00

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Product Information

The Ph.D.-C7C Phage Display Peptide Library is based on a combinatorial library of random peptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 10⁹ electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

Please click the link for Applications of the Ph.D. Phage Display Peptide Libraries.

Complexity: 1.2 x 10⁹ transformants.

Panning with a pentavalent peptide library displayed on plll.

System Components

The following reagents are supplied with this product:

	Store at (°C)	Concentration
E. coli K12 ER2738	-70
Streptavidin, lyophilized	-20
Biotin	-20	10 mM
Ph.D.™-C7C Phage Display Peptide Library	-20	1 x10<sup>13</sup> pfu/ml
Streptavidin, lyophilized	-20
Biotin	-20	10 mM
-28 glll Sequencing Primer (22-mer)	-20	1 pmol/μl
-96 glll Sequencing Primer (20-mer)	-20	1 pmol/μl

Product Categories:: Phage Display

Applications:: phage-display

Properties and Usage

Storage Temperature
-20°C
Related Products
Companion Products
Materials Sold Separately
- E. coli K12 ER2738
- Ph.D.™-C7C Phage Display Peptide Library
References
1. Sidhu, S.S. et al. (2003). Chembiochem.. 4, 14-25.
2. Azzazy, H.M. and Highsmith, W.E. (2002). Clin. Biochem.. 35, 425-445.
3. Rodi, D.J. et al. (2002). Curr. Opin. Chem. Biol.. 6, 92-96.
4. Parmley, S.F. and Smith, G.P. (1988). Gene. 73, 305-318.
5. Berggard, T. et al. (2002). J. Biol. Chem.. 277, 41954-41959.
6. Chaudhary, J. et al. (2001). Am. J. Physiol. Cell Physiol.. 280, C1027-1030.
7. Chen, L. and Sigler, P.B. (1999). Cell. 99, 757-768.
8. Biorn, A.C. et al. (2004). Biochemistry. 43, 1928-1938.
9. Ferrer, M. and Harrison, S.C. (1999). J. Virol.. 73, 5795-5802.
10. Ferrer, M. et al. (1999). J. Pept. Res.. 54, 32-42.
11. BouHamdan, M. et al. (1998). J. Biol. Chem.. 273, 8009-8016.
12. Whaley, S.R. et al. (2000). Nature. 405, 665-668.
13. Rozinov, M.N. and Nolan, G.P. (1998). Chem. Biol.. 5, 713-728.
14. Rodi, D.J. et al. (1999). J. Mol. Biol.. 285, 197-203.
15. Kraft, S. et al. (1999). J. Biol. Chem.. 274, 1970-1985.
16. Koolpe, M. et al. (2002). J. Biol. Chem.. 277, 46974-46979.
17. Mummert, M.E. et al. (2000). J. Exp. Med.. 192, 769-779.
18. Hetian, L. et al. (2002). J. Biol. Chem.. 277, 43137-43142.
19. White, S.J. et al. (2001). Hypertension. 37, 449-455.
20. Binetruy-Tournaire, R. et al. (2000). EMBO J.. 19, 1525-1533.
21. Kragler, F. et al. (2000). EMBO J.. 19, 2856-2868.
22. Gazouli, M. et al. (2002). J. Pharmacol. Exp. Ther.. 303, 627-632.
23. Romanczuk, H. et al. (1999). Hum. Gene Ther.. 10, 2615-2626.
24. Nicklin, S.A. et al. (2000). Circulation. 102,
25. Jost, P.J. et al. (2001). FEBS Lett.. 489, 263-269.
26. Rasmussen, U.B. et al. (2002). Cancer Gene Ther.. 9, 606-612.
27. Tinoco, L.W. et al. (2002). J. Biol. Chem.. 277, 36351-36356.
28. Stratmann, J. et al. (2002). J. Clin. Microbiol.. 40, 4244-4250.
29. Mourez, M. et al. (2001). Nat. Biotechnol.. 19, 958-961.
30. Lee, L. et al. (2002). Arthritis Rheum.. 46, 2109-2120.
31. Duerr, D.M. et al. (2004). J. Virol. Methods. 116, 177-180.

FAQs & Tech Tips
- FAQs
  Phage Library Choice
  
  I am using Ph.D.™ Phage display and the amplified phage titer is low.
  
  I am using Ph.D.™ Phage Display and the phage DNA templates do not yield readable sequence.
  
  I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
  
  I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
  
  When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
  
  When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
  
  I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
  
  Can a different bacterial strain be used with the Ph.D.™ Phage Display?
  
  Where can I find references for Ph.D.™ phage display libraries?
Protocols & Manuals
- Manuals
  The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].)
  
  manualE8120
- Protocols
  M13 Amplification
  
  M13 Titer Protocol
Other Tools & Resources
- Troubleshooting Guides
  Phage Display Troubleshooting Guide
- Interactive Tools
  NEBioCalculator
- Citations
  Matochko WL, Cory Li S, Tang SK, Derda R (2014) Prospective identification of parasitic sequences in phage display screens Nucleic Acids Res; 42(3), 1784-98. PubMedID: 24217917 , DOI: 10.1093/nar/gkt1104
  
  Nguyen KT, Adamkiewicz MA, Hebert LE, Zygiel EM, Boyle HR, Martone CM, Meléndez-Ríos CB, Noren KA, Noren CJ, Hall MF (2014) Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries Anal Biochem; 462C, 35-43. PubMedID: 24952360 , DOI: 10.1016/j.ab.2014.06.007
  
  Kaur K, Taneja NK, Dhingra S, Tyagi JS (2014) DevR (DosR) mimetic peptides impair transcriptional regulation and survival of Mycobacterium tuberculosis under hypoxia by inhibiting the autokinase activity of DevS sensor kinase BMC Microbiol; 14, 195. PubMedID: 25048654 , DOI: 10.1186/1471-2180-14-195
Quality & Safety
- Quality Control Assays
  
  Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.
- Specifications
  The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]
  
  E8120S_v1
- Certificate of Analysis
  The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]
  
  E8120S_v1_10007441
- Safety Data Sheets
  The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
  
  Ph.D.™-C7C Phage Display Peptide Library Kit
  
  E. coli K12 ER2738
  
  Ph.D.™-C7C Phage Display Peptide Library
  
  Streptavidin, lyophilized
  
  Biotin
  
  -28 glll Sequencing Primer (22-mer)
  
  -96 glll Sequencing Primer (20-mer)
Legal Information
- Legal And Disclaimers
  
  This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).
  
  While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.
  
  For more information about commercial rights, please contact NEB's Global Business Development team at gbd@neb.com.
  
  This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Applications

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Tools

Resources

Contact Information

Applications

Product Categories

You’ll be Thrilled to Pieces

Tools

Resources

Contact Information

Ph.D.™-C7C Phage Display Peptide Library Kit

Ordering Information

System Components

Storage Temperature

Companion Products

Materials Sold Separately

Also Recommended

Ph.D.™-12 Phage Display Peptide Library Kit

Ph.D.™ Peptide Display Cloning System

Ph.D.™-7 Phage Display Peptide Library Kit

Ph.D.™-C7C Phage Display Peptide Library Kit

Ordering Information

System Components

Storage Temperature

Companion Products

Materials Sold Separately

Also Recommended

Ph.D.™-12 Phage Display Peptide Library Kit

Ph.D.™ Peptide Display Cloning System

Ph.D.™-7 Phage Display Peptide Library Kit

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