Ph.D.™-C7C Phage Display Peptide Library Kit

Companion Products

• Ph.D.™-7 Phage Display Peptide Library Kit
• Ph.D.™ Peptide Display Cloning System
• Ph.D.™-12 Phage Display Peptide Library Kit

Materials Sold Separately

• E. coli K12 ER2738

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1. M13 Titer Protocol
2. M13 Amplification

1. Phage Library Choice
2. I am using Ph.D.™ Phage display and the amplified phage titer is low.
3. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield readable sequence.
4. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
5. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
6. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
7. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
8. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
9. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
10. Where can I find references for Ph.D.™ phage display libraries?

• Phage Display Troubleshooting Guide

• Matochko WL, Cory Li S, Tang SK, Derda R (2014) Prospective identification of parasitic sequences in phage display screens Nucleic Acids Res; 42(3), 1784-98. PubMedID: 24217917 , DOI: 10.1093/nar/gkt1104
• Nguyen KT, Adamkiewicz MA, Hebert LE, Zygiel EM, Boyle HR, Martone CM, Meléndez-Ríos CB, Noren KA, Noren CJ, Hall MF (2014) Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries Anal Biochem; 462C, 35-43. PubMedID: 24952360 , DOI: 10.1016/j.ab.2014.06.007
• Kaur K, Taneja NK, Dhingra S, Tyagi JS (2014) DevR (DosR) mimetic peptides impair transcriptional regulation and survival of Mycobacterium tuberculosis under hypoxia by inhibiting the autokinase activity of DevS sensor kinase BMC Microbiol; 14, 195. PubMedID: 25048654 , DOI: 10.1186/1471-2180-14-195

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

• E8120S_v1
• E8120S_v2

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

• E8120S_v1_10007441
• E8120S_v1_10026162
• E8120S_v2_10043274

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

• E. coli K12 ER2738
• Ph.D.™-C7C Phage Display Peptide Library
• Streptavidin, lyophilized
• Biotin
• -28 glll Sequencing Primer (22-mer)
• -96 glll Sequencing Primer (20-mer)

This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB).

While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications.

For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.