The Ph.D.-12™ Phage Display Peptide Library Kit contains a tube of the Ph.D.-12 Phage Library, protein reagents for a control reaction and sequencing primer stocks. The Ph.D.-12 library is a combinatorial library of random 12-mer peptides fused to a minor coat protein (pIII) of M13 phage. The displayed peptide is expressed at the N-terminus of pIII. The library consists of approximately 109 electroporated (i.e., unique) sequences.
The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.Citations for Phage Display
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|E. coli K12 ER2738||-70|
|Ph.D.™-12 Phage Display Peptide Library||-20||1 x1013 pfu/ml|
|-28 glll Sequencing Primer (22-mer)||-20||1 pmol/µl|
|-96 glll Sequencing Primer (20-mer)||-20||1 pmol/µl|
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