Ph.D.™-12 Phage Display Peptide Library Kit


The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

Citations for Phage Display

Panning with a pentavalent peptide library displayed on pIII.

Kit Components

The following reagents are supplied with this product:

Store at (°C)Concentration
Streptavidin, lyophilized-20
Biotin-2010 mM
E. coli K12 ER2738-70
-28 glll Sequencing Primer (22-mer)-201 pmol/μl
-96 glll Sequencing Primer (20-mer)-201 pmol/μl
Ph.D.™-12 Phage Display Peptide Library-201 x1013 pfu/ml
Streptavidin, lyophilized-20
Biotin-2010 mM

Properties and Usage


  1. Sidhu, S.S. et al. (2003). Chembiochem. 4, 14-25.
  2. Azzazy, H.M. and Highsmith, W.E. (2002). Clin. Biochem.. 35, 425-445.
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  9. Biorn, A.C. et al. (2004). Biochemistry. 43, 1928-1938.
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  11. BouHamdan, M. et al. (1998). J. Biol. Chem.. 273, 8009-8016.
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  1. Phage Library Choice
  2. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
  3. No plaques are visible when titering using the Ph.D.™ Phage Display kit.
  4. I am using Ph.D.™ Phage display and the amplified phage titer is low.
  5. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield readable sequence.
  6. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
  7. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
  8. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
  9. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
  10. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
  11. Where can I find references for Ph.D.™ phage display libraries?


  1. M13 Amplification
  2. M13 Titer Protocol


The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

Troubleshooting Guides

Interactive Tools


  • Ohnishi T., Yanazawa M., Sasahara T., Kitamura Y., Hiroaki H., Fukazawa Y., Kii I., Nishiyama T., Kakita A., Takeda H., Takeuchi A., Arai Y., Ito A., Komura H., Hirao H., Satomura K., Inoue M., Muramatsu S., Matsui K., Tada M., Sato M., Saijo E., Shigemitsu Y., Sakai S., Umetsu Y., Goda N., Takino N., Takahashi H., Hagiwara M., Sawasaki T., Iwasaki G., Nakamura Y., Nabeshima Y., Teplow D.B., Hoshi M. (2015). Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. Proc Natl Acad Sci U S A. 112, E4465-74. PubMedID: 26224839, DOI: 10.1073/pnas.1421182112
  • Shires K, Shankland I, Mowla S, Njikan S, Jaymacker J, Novitzky N (2014). Serine and proline-rich ligands enriched via phage-display technology show preferential binding to BCR/ABL expressing cells Hematol Oncol Stem Cell Ther. 7(1), 32-40. PubMedID: 24480037, DOI: 10.1016/j.hemonc.2014.01.001
  • Fatima A, Wang H, Kang K, Xia L, Wang Y, Ye W, Wang J, Wang X. (2014). Development of VHH Antibodies against Dengue Virus Type 2 NS1 and Comparison with Monoclonal Antibodies for Use in Immunological Diagnosis PLoS One. 9(4), e95263. PubMedID: 24751715, DOI: 10.1371/journal.pone.0095263
  • Baumgartner J, Carillo MA, Eckes KM, Werner P, Faivre D. (2014). Biomimetic Magnetite Formation From Biocombinatorial Approaches to Mineralization Effects Langmuir. 30(8), 2129-36. PubMedID: 24499323, DOI: 10.1021/la404290c
  • McGuire MJ, Gray BP, Li S, Cupka D, Byers LA, Wu L, Rezaie S, Liu YH, Pattisapu N, Issac J, Oyama T, Diao L, Heymach JV, Xie XJ, Minna JD, Brown KC (2014). Identification and Characterization of a Suite of Tumor Targeting Peptides for Non-Small Cell Lung Cancer Sci Rep. 4, 4480. PubMedID: 24670678, DOI: 10.1038/srep04480
  • Maneewatch S, Adisakwattana P, Chaisri U, Saengjaruk P, Srimanote P, Thanongsaksrikul J, Sakolvaree Y, Poungpan P, Chaicumpa W (2014). Therapeutic epitopes of Leptospira LipL32 protein and their characteristics Protein Eng Des Sel. 27(5), 135-44. PubMedID: 24760832, DOI: 10.1093/protein/gzu006
  • Grin I, Hartmann MD, Sauer G, Hernandez Alvarez B, Schütz M, Wagner S, Madlung J, Macek B, Felipe-Lopez A, Hensel M, Lupas A, Linke D (2014). A trimeric lipoprotein assists in trimeric autotransporter biogenesis in enterobacteria J Biol Chem. 289(11), 7388-98. PubMedID: 24369174, DOI: 10.1074/jbc.M113.513275
  • Matochko WL, Cory Li S, Tang SK, Derda R (2014). Prospective identification of parasitic sequences in phage display screens Nucleic Acids Res. 42(3), 1784-98. PubMedID: 24217917, DOI: 10.1093/nar/gkt1104
  • Kaur K, Taneja NK, Dhingra S, Tyagi JS (2014). DevR (DosR) mimetic peptides impair transcriptional regulation and survival of Mycobacterium tuberculosis under hypoxia by inhibiting the autokinase activity of DevS sensor kinase BMC Microbiol. 14, 195. PubMedID: 25048654, DOI: 10.1186/1471-2180-14-195
  • Nguyen KT, Adamkiewicz MA, Hebert LE, Zygiel EM, Boyle HR, Martone CM, Meléndez-Ríos CB, Noren KA, Noren CJ, Hall MF (2014). Identification and characterization of mutant clones with enhanced propagation rates from phage-displayed peptide libraries Anal Biochem. 462C, 35-43. PubMedID: 24952360, DOI: 10.1016/j.ab.2014.06.007

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.