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  • Ph.D.™-12 Phage Display Peptide Library Kit

    Description

    The Ph.D.-12 Phage Display Peptide Library is based on a combinatorial library of random dodecapeptides fused to a minor coat protein (pIII) of M13 phage (1–6). The displayed peptide (12-mer) is expressed at the N-terminus of pIII, i.e., the first residue of the mature protein is the first randomized position. The peptide is followed by a short spacer (Gly-Gly-Gly-Ser) and then the wild-type pIII sequence. The library consists of approximately 109 electroporated sequences amplified once to yield approximately 100 copies of each sequence in 10 µl of the supplied phage.

    Citations for Phage Display



    Panning with a pentavalent peptide library displayed on pIII.

    Kit Components

    The following reagents are supplied with this product:

    Store at (°C)Concentration
    Streptavidin, lyophilized-20
    Biotin-2010 mM
    E. coli K12 ER2738-70
    -28 glll Sequencing Primer (22-mer)-201 pmol/μl
    -96 glll Sequencing Primer (20-mer)-201 pmol/μl
    Ph.D.™-12 Phage Display Peptide Library-201 x1013 pfu/ml
    Streptavidin, lyophilized-20
    Biotin-2010 mM

    Properties and Usage

    References

    1. Sidhu, S.S. et al. (2003). Chembiochem. 4, 14-25.
    2. Azzazy, H.M. and Highsmith, W.E. (2002). Clin. Biochem.. 35, 425-445.
    3. Rodi, D.J. et al. (2002). Curr. Opin. Chem. Biol.. 6, 92-96.
    4. Parmley, S.F. and Smith, G.P. (1988). Gene. 73, 305-318.
    5. Berggard, T. et al. (2002). J. Biol. Chem.. 277, 41954-41959.
    6. Chaudhary, J. et al. (2001). Am. J. Physiol. Cell Physiol.. 280
    7. Chen, L. and Sigler, P.B. (1999). Cell. 99, 757-769.
    8. Ferrer, M. and Harrison, S.C. (1999). J. Virol.. 73, 5795-5802.
    9. Biorn, A.C. et al. (2004). Biochemistry. 43, 1928-1938.
    10. Ferrer, M. et al. (1999). J. Pept. Res.. 54, 32-42.
    11. BouHamdan, M. et al. (1998). J. Biol. Chem.. 273, 8009-8016.
    12. Whaley, S.R. et al. (2000). Nature. 405, 665-668.
    13. Rozinov, M.N. and Nolan, G.P. (1998). Chem. Biol.. 5, 713-728.
    14. Rodi, D.J. et al. (1999). J. Mol. Biol.. 285, 197-203.
    15. Kraft, S. et al. (1999). J. Biol. Chem.. 274, 1979-1985.
    16. Koolpe, M. et al. (2002). J. Biol. Chem.. 277, 46974-46979.
    17. Mummert, M.E. et al. (2000). J. Exp. Med.. 192, 769-779.
    18. Hetian, L. et al. (2002). J. Biol. Chem.. 277, 43137-43142.
    19. White, S.J. et al. (2001). Hypertension. 37, 449-455.
    20. Binetruy-Tournaire, R. et al. (2000). EMBO J.. 19, 1525-1533.
    21. Kragler, F. et al. (2000). EMBO J.. 19, 2856-2868.
    22. Gazouli, M. et al. (2002). J. Pharmacol. Exp. Ther.. 303, 627-632.
    23. Romanczuk, H. et al. (1999). Hum. Gene Ther.. 10, 2615-2626.
    24. Nicklin, S.A. et al. (2000). Circulation. 102, 231-237.
    25. Jost, P.J. et al. (2001). FEBS Lett.. 489, 263-269.
    26. Rasmussen, U.B. et al. (2002). Cancer Gene Ther.. 9, 606-612.
    27. Tinoco, L.W. et al. (2002). J. Biol. Chem.. 277, 36351-36356.
    28. Stratmann, J. et al. (2002). J. Clin. Microbiol.. 40, 4244-4250.
    29. Mourez, M. et al. (2001). Nat. Biotechnol.. 19, 958-961.
    30. Lee, L. et al. (2002). Arthritis Rheum.. 46, 2109-2120.
    31. Duerr, D.M. et al. (2004). J. Virol. Methods. 116, 177-180.

    FAQs

    1. Phage Library Choice
    2. Can a different bacterial strain be used with the Ph.D.™ Phage Display?
    3. No plaques are visible when titering using the Ph.D.™ Phage Display kit.
    4. I am using Ph.D.™ Phage display and the amplified phage titer is low.
    5. I am using Ph.D.™ Phage Display and the phage DNA templates do not yield readable sequence.
    6. I am using Ph.D.™ Phage Display and the sequencing templates do not run where they should on a gel.
    7. I am using Ph.D.™ Phage Display and after 4 or more rounds of panning all clones are wild-type phage (white plaques).
    8. When performing an experiment using Ph.D.™ Phage Display, the ELISA indicates that background binding to the plate is as high as binding to the target.
    9. When using the Ph.D.™ Phage Display, panning yielded a consensus sequence, but no ELISA signal.
    10. I am using Ph.D.™ Phage Display and the streptavidin control experiment did not yield the HPQ consensus sequence.
    11. Where can I find references for Ph.D.™ phage display libraries?

    Protocols

    1. M13 Amplification
    2. M13 Titer Protocol

    Manuals

    The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name these document files: manual[Catalog Number].

    Troubleshooting Guides

    Interactive Tools

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.