Anti-MBP Magnetic Beads

Description

An affinity matrix for small-scale isolation and purification of (MBP) fusion proteins. Monoclonal anti-MBP is covalently coupled to 1 µm nonporous paramagnetic particles through a linkage that is stable and leak resistant over a wide pH range. This permits immunomagnetic isolation of MBP-fusion proteins from cell culture extracts. Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins that specifically interact with the immobilized MBP fusion protein.

Properties and Usage

Storage Temperature

4°C

Storage Conditions

0.05% Tween® 20
0.02% sodium azide
1X PBS
pH 7.4 @ 25°C

Binding Capacity

5 μg MBP-paramyosin Dsal fusion protein / mg Anti-MBP beads.

Can be Regenerated

yes

Shipping Notes

  • 4°C

Protocols

  1. Isolation of MBP-fusion protein using Anti-MBP Magnetic Beads

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

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Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.