Chitin Magnetic Beads

 

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).

  • Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
  • The matrix can be regenerated without loss of binding capacity
  • Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
  • Binding capacity of 2 mg chitin binding domain protein/ml bed volume
  • Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
  • Removal of CBD-tag during elution typically yields highly pure, native protein

Ordering Information

E8036S_pdp_800x450
  • 5 ml
    $122.00
  • 25 ml
    $491.00
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