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  • Anti-CBD Monoclonal Antibody

    Description

    Anti-CBD Monoclonal Antibody is a murine anti-chitin binding domain (CBD) antibody, isotype IgG1.

    IMPACT Citations

    Properties and Usage

    Antibody Type

    Monoclonal

    Isotype

    Mouse Isotype IgG1

    Storage Temperature

    -20°C

    Notes

    1. Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3 or an equivalent antimicrobial agent.

    References

    1. Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H. and Xu, M.-Q. (1997). Gene. 192, 271-281.
    2. Watanabe, T., Ito, Y., Yamada, T., Hashimoto, M., Sekine, S. and Tanaka, H. (1994). J. Bacteriol. 176, 4465-4472.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

    Quality Control

    Quality Assurance Statement

    • In an ELISA assay, a dilution of 1/1,000 gives a signal of at least 20% of the maximum signal using high concentrations of antibody. The same dilution gives a strong signal when used to detect chitin-binding domain fusion protein in Western blots developed with the Phototope-HRP Western Blot Detection System (Cell Signaling Technology). This antibody does not cross-react with other E. coli proteins.

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Physical Purity (HPLC):
      The purity of the product is determined by HPLC analysis.

    Safety Data Sheet

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.