• My NEB
  • Print
  • PDF
  • Anti-CBD Monoclonal Antibody

    Description

    Anti-CBD Monoclonal Antibody is a murine anti-chitin binding domain (CBD) antibody, isotype IgG1.

    Properties and Usage

    Antibody Type

    Monoclonial

    Isotype

    Mouse Isotype IgG2a

    Storage Temperature

    -20°C

    Quality Control

    Quality Assurance Statement

    • In an ELISA assay, a dilution of 1/2000 gives a signal of at least 20% of the maximum signal using concentrated antibody (detected with OPD as the substrate). The same 1/2000 dilution gives a strong signal when used to detect maltose-binding protein in Western blots developed with a variety of detection systems. This antibody does not cross-react with other E. coli proteins.

    Quality Control Assays

    The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page.
    • Physical Purity (HPLC):
      The purity of the product is determined by HPLC analysis.

    Notes

    1. Store at -20°C undiluted. May be stored at 4°C diluted in buffer containing 1 mM NaN3 or an equivalent antimicrobial agent.

    References

    1. Chong, S., Mersha, F.B., Comb, D.G., Scott, M.E., Landry, D., Vence, L.M., Perler, F.B., Benner, J., Kucera, R.B., Hirvonen, C.A., Pelletier, J.J., Paulus, H. and Xu, M.-Q. (1997). Gene. 192, 271-281.
    2. Watanabe, T., Ito, Y., Yamada, T., Hashimoto, M., Sekine, S. and Tanaka, H. (1994). J. Bacteriol. 176, 4465-4472.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.