Anti-M13 pIII Monoclonal Antibody

Description

Anti-M13 pIII Monoclonal Antibody (mouse isotype IgG2a) is derived from BALB/c mice immunized with the C-terminal half of M13 coat protein III (residues 259-406). Monoclonal anti-M13 pIII recognizes nonreduced and denatured reduced forms of wild type pIII or pIII fusions in immunoblotting and ELISA. ELISA assay against whole M13 phage not recommended since the epitope lies in a region not accessible on intact M13 phage virions.

Advantages and Features

Applications

  • Recombinant protein domains such as antibody fragments can be fused to M13 coat protein pIII and displayed on phage using phagemid-based systems (1), rather than the phage-based system utilized in the Ph.D. libraries. Anti-M13 pIII Monoclonal Antibody can be used to determine fusion protein expression levels both in E.coli and on the M13 virion by immunoblot analysis.

Properties and Usage

Antibody Type

Monoclonal

Isotype

Mouse Isotype IgG2a

Recommended Dilution for Western Blot

1:1000

Storage Temperature

-20°C

Notes

  1. Store undiluted

Protocols

  1. Western Blot (NEB #E8033)

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.

Interactive Tools

Quality Control

Quality Assurance Statement

  • A 1:1,000 dilution of the antibody is tested for its ability to detect 107 pfu M13 by Western blot.

Safety Data Sheet

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Datacards

The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.