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  • Anti-M13 pIII Monoclonal Antibody

    Description

    Anti-M13 pIII Monoclonal Antibody (mouse isotype IgG2a) is derived from BALB/c mice immunized with the C-terminal half of M13 coat protein III (residues 259-406). Monoclonal anti-M13 pIII recognizes nonreduced and denatured reduced forms of wild type pIII or pIII fusions in immunoblotting and ELISA. ELISA assay against whole M13 phage not recommended since the epitope lies in a region not accessible on intact M13 phage virions.

    Advantages and Features

    Applications

    • Recombinant protein domains such as antibody fragments can be fused to M13 coat protein pIII and displayed on phage using phagemid-based systems (1), rather than the phage-based system utilized in the Ph.D. libraries. Anti-M13 pIII Monoclonal Antibody can be used to determine fusion protein expression levels both in E.coli and on the M13 virion by immunoblot analysis.

    Properties and Usage

    Antibody Type

    Monoclonial

    Isotype

    Mouse Isotype IgG2a

    Target Antigen

    Internal portion of M13 coat protein III

    Recommended Dilution for Western Blot

    1:1000

    Storage Temperature

    -20°C

    Quality Control

    Quality Assurance Statement

    • A dilution of 1/1,000 detected as few as 107 M13 CFU electrophoresed and transferred onto Immobolin PVDF membrane in an immunoblot assay.

    Supporting Documents

    Material Safety Datasheets

    The following is a list of Material Safety Data Sheets (MSDS) that apply to this product to help you use it safely. The following file naming structure is used to name these document files: [Product Name] MSDS. For international versions please contact us at info@neb.com.

    Datacards

    The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at info@neb.com or fill out the Technical Support Form for appropriate document.